Conformational change in transfer RNA is an early indicator of acute cellular damage
| Project/Area Number |
26860624
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | tRNA / 転移RNA / 急性腎障害 / 修飾核酸 / 1-メチルアデノシン / シュードウリジン / 酸化ストレス / バイオマーカー / rRNA / 修飾塩基 / ｔRNA / 慢性腎臓病 / 急性腎傷害 / 核酸 |
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| Outline of Final Research Achievements |
We revealed that the detection of 1-methyladenosine, which is a modified nucleoside containing in transfer RNA, is a useful new biomarker for diagnosis of acute kidney injury (Mishima et al. JASN, 2014). In addition, we showed that circulating 1-methyladenosine levels are significantly associated with the mortality in the general population using the sample of Ohasama cohort study. Therefore, tRNA damage reflects early oxidative stress damage, and detection of tRNA damage may be a useful tool for identifying organ damage and forming a clinical prognosis. Furthermore, we developed a method for detecting RNA modifications called immuno-northern blotting analysis and confirmed its various capabilities (Mishima et al. PLoS ONE, 2015).
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides2015
Author(s)
Mishima E, Jinno D, Akiyama Y, Itoh K, Nankumo S, Shima H, Kikuchi K, Takeuchi Y, Elkordy A, Suzuki T, Niizuma K, Ito S, Tomioka Y, Abe T
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Journal Title
PLos One
Volume: 10
Issue: 11
Pages: e0143756-e0143756
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases2015
Author(s)
Suzuki T, Yamaguchi H, Kikusato M, Matsuhashi T, Matsuo A, Sato T, Oba Y, Watanabe S, Minaki D, Saigusa D, Shimbo H, Mori N, Mishima E, Shima H, Akiyama Y, Takeuchi Y, Yuri A, Kikuchi K, Toyohara T, Suzuki C, Kohzuki M, Anzai J, Mano N, Kure S, Yanagisawa T, Tomioka Y, Toyomizu M, Ito S, Osaka H, Hayashi K, Abe T
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Journal Title
The Tohoku Journal of Experimental Medicine
Volume: 236
Issue: 3
Pages: 225-232
DOI
NAID
ISSN
0040-8727, 1349-3329
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Alteration of the Intestinal Environment by Lubiprostone Is Associated with Amelioration of Adenine-Induced CKD2014
Author(s)
Eikan Mishima, Shinji Fukuda, Hisato Shima, Akiyoshi Hirayama, Yasutoshi Akiyama, Yoichi Takeuchi, Noriko N Fukuda, Takehiro Suzuki, Chitose Suzuki, Akinori Yuri, Koichi Kikuchi, Yoshihisa Tomioka, Sadayoshi Ito, Tomoyoshi Soga, and Takaaki Abe
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Journal Title
J Am Soc Nephrol
Volume: 26
Issue: 8
Pages: 1-8
DOI
Related Report
Peer Reviewed
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