| Project/Area Number |
26861312
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University |
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Principal Investigator |
KAKU Hiroshi 千葉大学, 医学部附属病院, 医員 (40456059)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 絨毛性疾患 / 胚細胞性腫瘍 / 胞状奇胎 / CISH / 免疫染色 |
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| Outline of Final Research Achievements |
Precise diagnosis of hydatidiform mole is important because some extent of molar patients would be developing into gestational trophoblastic neoplasia. The aim of this study was the establishment of molecular pathological diagnosis with p57KIP2 immunohistochemistry and Chromogenic in situ Hybridization (CISH). The immune staining of p57KIP2 determined androgenetic hydatidiform moles. Otherwise, CISH discriminated the triploidy of partial hydatidiform moles from biparental diploid abortion.
Seventy-five percent of cases demonstrated the appropriated CISH signals, although twenty-five percent of cases did not indicate the signals. To get the stable signal, the methods of the fixation of the villous sample could be critical. Further investigation would be necessary.
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