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Biochemical Research on the Gonadotropin Receptor in Relation to Its Function

Research Project

Project/Area Number 63570780
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKobe University, School of Medicine

Principal Investigator

KATAYAMA K.  Kobe Univ. Medicine, Assistant., 医学部, 助手 (70112084)

Co-Investigator(Kenkyū-buntansha) MOCHIZUKI M.  Kobe Univ. Medicine, Professor, 医学部, 教授 (80030922)
西村 隆一郎  神大, 医学部附属病院, 助手 (00127383)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsLH / hCG receptor / cross linker / hormone-receptor complex / alpha-subunit / beta-subunit / progesterone / amnti-progestercne / antiーprogesterone / LH@hCG receptor / Western blot法 / 卵巣LH / hCGレセプターの構造 / affinity label / hCG糖鎖
Research Abstract

The structure of LH/HCG receptor of rat luteal cells was investigated using different two types of cross linkers. Rat luteal cells obtained from Parlow rats were incubated with hCG whose alpha-subunit or beta-subunit is labeled with ^<125>I. hCG-receptor complex was cross linked from inner side by Photoaffinity labeling (ABG) and from outer side by SES. After solubilized with SDS, reduced with DTT, the cross-linked samples were applied on SDS-PAGE and observed by autoradiography. When ^<125>I-alpha+beta was used, 4 complexes, A(MW 136k), B(105k), C(78k), D(72k) were found by ARG and 3 complexes, A(136k), B(105k), C(78k) by SES. When alpha+^<125>I-beta was used, 4 complexes A(136k), B(116k), C(90k), D(72k) were found by ABG and strong A(136k) and weak D(72k) by SES. From these results it was suggested that LH/hCG receptor is composed of several proteins and that the binding mechanism of hCG to its receptor is such that the alpha subunit is contained within the beta subunit.
In the following experiments we investigated the effects of progesterone or anti-progesterone on the structure of the LH/hCG receptor. Specific binding capacity of the ovaries to LH/hCG were studied in D7 pregnant rats treated with RU 1mg/kg alone (RU group) or with both RU 1mg/kg and progesterone 50mg/kg (RU+P group). The specific binding, of LH/hCG to ovarian homogenates decreased significantly after 72 hours in the RU group. But in the RU+P grolip, the specific bindings were kept at the sante levels as the controls. Scatcliard analysis of these restilts disclosed that in the RU group, both affinity and numbers of receptors decreased compared to the controls, and that in the RU+P group only affinity decreased transiently and afterwards recovered quickly. From these results it was concluded that the structure of LH/hCG receptors of rat luteal cells are not changed by propesterone or anti--progesterone but changed in those number and affinity.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Nishimura,R.,Kitajuma,T.,Hasegawa,K.,Takeuchi,K.and Mochizuki,M.: "Molecular Forms of Human Chorionic Gonadotropin in Choriocarcinoma Serum and Urine" Jpn.J.Cancer R.80. 968-974 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 山辺晋吾、片山和明、望月眞人: "妊娠黄体の機能に対するanti-progesterone剤の作用とprogesteroneの意義" 日本内分泌学会雑誌. 65. 497-511 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 阿江孝、片山和明、望月眞人: "無月経患者における甲状腺ホルモン動態の解析ー特に体重減少性無月経患者における性機能との関連性" 日本不妊学会雑誌. 35. 1-10 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishimura, R., Kitajima, T., Hasegawa, K., Takeuchi, K. and Mochizuki, M.: "Molewlar Forms of Human Chorionic Gonadotropin in Choriocarcinoma Seram and Urine" Jpn.J.Cancer.Res.80. 968-974 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamabe, S., Katayama, K. and Mochizuki, M.: "The Effect of RU486 and Progesterone on Luteal Function during Pregnancy" Folia Endocrinol 65, 497-511, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ae, T., Katayama, K. and Mochizuki, M.: "The Levels of Thyroid Hormanes in Patients with Menstrual Disorders" Jap.J.Fert. & Ster.35, 1-10, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishimura,R.,Kitajima,T.Hasegawa,K.,Takeuchi,K.and Mochizuki,M.: "Molecular Forms of Human Chorionic Gonadotropin in Choriocarcinoma Serum and Urine" Jpn.J.Cancer R.80. 968-974 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 山辺晋吾,片山和明,望月眞人: "妊娠黄体の機能に対するantiーprogesterone剤の作用とprogesteroneの意義" 日本内分泌学会雑誌. 65. 497-511 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 阿江孝,片山和明,望月眞人: "無月経患者における甲状腺ホルモン動態の解析ー特に体重減少性無月経患者における性機能との関連性" 日本不妊学会雑誌. 35. 1-10 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 西村隆一郎: 日産婦誌. 38. 261-262 (1986)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ryuichiro Nishimura.: Proc.Natl.Acad.Sci.83. 6327-6331 (1986)

    • Related Report
      1988 Annual Research Report
  • [Publications] 山辺晋吾: 日内分泌誌. 5. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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