2013 Fiscal Year Final Research Report
Molecular regulation of ROS signal receptor proteins: S-guanylation proteomics
| Project Area | Signaling functions of reactive oxygen species |
|---|
| Project/Area Number |
20117005
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Tohoku University (2012-2013)
Kumamoto University (2008-2011) |
|---|
Principal Investigator |
AKAIKE Takaaki 東北大学, 医学(系)研究科(研究院), 教授 (20231798)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAWA Tomohiro 東北大学, 医学系研究科, 准教授 (30284756)
IWAI Sumio 鹿児島大学, 農学部, 教授 (50295276)
IHARA Hideshi 大阪府立大学, 理学系研究科, 准教授 (60254447)
|
|---|
| Project Period (FY) |
2008-11-13 – 2013-03-31
|
| Keywords | 活性酸素 / シグナル伝達 / プロテオミクス / 酸化ストレス / 生理活性 |
|---|
| Research Abstract |
8-Nitro-cGMP functions as a second messenger for ROS signaling via protein S-guanylation, a unique posttranslational protein thiol modification. In this study, new methodology was developed for identification of S-guanylation proteome. Several ROS sensor proteins were identified and examined for their impacts of S-guanylation on cellular functions. We further identified that metabolic regulation n of 8-nitro-cGMP via sulfhydration to form 8-SH-cGMP mediated by reactive sulfur species generated by cys
tathione beta-synthase and cystathione gamma-lyase systems.
|
-
[Journal Article] SIRT7 controls hepatic lipid metabolism by regulating the ubiquitin-proteasome pathway2014
Author(s)
Yoshizawa T, Karim Md. F, Sato Y, Senokuchi T, Miyata K, Fukuda T, Go C, Tasaki M, Uchimura K, Kadomatsu T, Tian Z, Smolka C, Sawa T, Kakeya M, Tomizawa K, Ando Y, Araki E, Akaike T, Braun T, Oike Y, Bober E, and Yamagata K
-
Journal Title
Cell Metab
Volume: 9
Pages: 712-721
DOI
Peer Reviewed
-
-
[Journal Article] Angiopoietin-like protein 2 accelerates carcinogenesis by activating chronic inflammation and oxidative stress2014
Author(s)
Aoi J, Endo M, Kadomatsu T, Miyata K, Ogata A, Horiguchi H, Odagiri H, Masuda T, Fukushima S, Jinnin M, Hirakawa S, Sawa T, Akaike T, Ihn H and Oike Y
-
Journal Title
Mol. Cancer Res
Volume: 12
Pages: 239-249
DOI
Peer Reviewed
-
-
[Journal Article] S-nitrosylated α-1-acid glycoprotein kills drug-resistant bacteria and aids survival in sepsis2013
Author(s)
Watanabe K, Ishima Y, Akaike T, Sawa T, Kuroda T, Ogawa W, Watanabe H, Suenaga A, Kai T, Otagiri M, and Maruyama T
-
Journal Title
FASEB J
Volume: 27
Pages: 391-398
DOI
Peer Reviewed
-
-
-
-
[Journal Article] Endogenous nitrated nucleotide is a key mediator of autophagy and innate defense against bacteria2013
Author(s)
Ito C, Saito Y, Nozawa T, Fujii S, Sawa T, Inoue H, Matsunaga T, Khan S, Akashi S, Hashimoto R, Aikawa C, Takahashi E, Sagara H, Komatsu M, Tanaka K, Akaike T, Nakagawa I. and Arimoto H
-
Journal Title
Mol. Cell
Volume: 52
Pages: 794-804
DOI
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Anks4b, a novel target of HNF4α interacts with GRP78 and regulates endoplasmic reticulum stress-induced apoptosis in pancreatic β-cells2012
Author(s)
Sato Y, Hatta M, Karim MF, Sawa T, Wei F-Y, Sato S, Magnuson MA, Gonzalez FJ, Tomizawa K, Akaike T, Yoshizawa T, and Yamagata K
-
Journal Title
J. Biol. Chem
Volume: 287
Pages: 23236-23245
DOI
Peer Reviewed
-
[Journal Article] S-Guanylation of human serum albumin is a unique posttranslational modification and results in a novel class of antibacterial agents2012
Author(s)
Ishima Y, Hoshino H, Shinagawa T, Watanabe K, Akaike T, Sawa T, Kragh-Hansen U, Kai T, Watanabe H, Maruyama T, and Otagiri M
-
Journal Title
J. Pharm. Sci
Volume: 101
Pages: 3222-3229
DOI
Peer Reviewed
-
-
[Journal Article] Hydrogen sulfide anion regulates redox signaling via electrophile sulfhydration2012
Author(s)
Nishida M, Sawa T, Kitajima N, Ono K, Inoue H, Ihara H, Motohashi H, Yamamoto M, Suematsu M, Kurose H, van der Vliet A, Freeman BA, Shibata T, Uchida K, Kumagai Y, and Akaike T
-
Journal Title
Nature Chem. Biol
Volume: 8
Pages: 714-724
DOI
Peer Reviewed
-
-
-
[Journal Article] Vascular responses to 8-nitro-cyclic GMP in nondiabetic and diabetic mice2011
Author(s)
Tokutomi Y, Kataoka K, Yamamoto E, Nakamura T, Hisato F, Nako H, Toyama K, Dong YF, Ahmed KA, Sawa T, Akaike T, and Kim-Mitsuyama S
-
Journal Title
Br. J. Pharmacol
Volume: 162
Pages: 1884-1893
DOI
Peer Reviewed
-
-
-
[Journal Article] Cellular uptake mechanisms and responses to NO transferred from mono- and poly-S-nitrosated human serum albumin2011
Author(s)
Ishima Y, Hoshino H, Shinagawa T, Watanabe K, Akaike T, Sawa T, Kragh-Hansen U, Kai T, Watanabe H, Maruyama T, and Otagiri M
-
Journal Title
Free Radic. Res
Volume: 45
Pages: 1196-1206
DOI
Peer Reviewed
-
-
-
-
[Journal Article] The critical role of nitric oxide signaling, via proteinS-guanylation and nitrated cyclic GMP, in the antioxidant adaptive response2010
Author(s)
Fujii S, Sawa T, Ihara H, Tong KI, Ida T, Okamoto T, Ahmed KA, Ishima Y, Motohashi H, Yamamoto M, and Akaike T
-
Journal Title
J. Biol. Chem
Volume: 285
Pages: 23970-23984
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
[Journal Article] Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling
Author(s)
Ida T, Sawa T, Ihara H, Tsuchiya Y, Watanabe Y, Kumagai Y, Suematsu M, Motohashi H, Fujii S, Matsunaga T, Yamamoto M, Ono K, Devarie-Baez N O, Xian M, Fukuto J M, and Akaike T
-
Journal Title
Natl. Acad. Sci. USA
Volume: (in press)
DOI
Peer Reviewed
-
[Journal Article] Promotion of atherosclerosis by Helicobacter cinaedi infection that involves macrophage-driven proinflammatory responses
Author(s)
Khan S, Rahman H N A, Okamoto T, Matsunaga T, Fujiwara Y, Sawa T, Yoshitake J, Ono K, Ahmed K A, Rahaman Md M, Oyama K, Takeya M, Ida T, Kitamura Y, Fujii S, and Akaike T
-
Journal Title
Sci. Rep.
Volume: (in press)
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
