2015 Fiscal Year Final Research Report
Evaluation of cellular functions by imaging cellular nucleic acids
| Project Area | Molecular Science for Nanomedicine |
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| Project/Area Number |
23107007
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Tokyo Institute of Technology (2013-2015)
Kyushu University (2011-2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA NAOHIKO 東京工業大学, 大学院生命理工学研究科, 助教 (10423972)
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| Project Period (FY) |
2011-04-01 – 2016-03-31
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| Keywords | カチオン性高分子 / ＤＮＡ / 核酸酵素 / ペプチド / 脂質膜 / グラフト共重合体 / 高分子電解質複合体 |
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| Outline of Final Research Achievements |
In this study, we showed that the cationic copolymer also enhances activity of an MNAzyme-type DNA sensor derived from the 10-23 DNAzyme and increased MNAzyme sensitivity. Furthermore, the copolymer enabled us to shorten the substrate-binding arms of the MNAzyme, decreasing the optimum temperature of the MNA assay from 50 °C to physiological temperature.
It was also demonstrated that the copolymer enhanced function of membrane disrupting peptides by facilitating their folding. Effective cytosolic delivery of macromolecular substances would be possible by using peptide/copolymer complex.
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| Free Research Field |
生体機能性高分子
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