The roles of receptors for lipid mediators in inflammation, immunity and barrier function

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04708
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Juntendo University
• Principal Investigator: Yokomizo Takehiko 順天堂大学, 医学(系)研究科(研究院), 教授 (60302840)
• Co-Investigator(Renkei-kenkyūsha): Okuno Toshiaki 順天堂大学, 大学院医学研究科, 准教授 (60361466) ; Saeki Kazuko 順天堂大学, 大学院医学研究科, 准教授 (00553273) ; Koga Tomoaki 順天堂大学, 大学院医学研究科, 助教 (30615092)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 生理活性脂質 / 生体分子 / シグナル伝達 / バリア機能 / アラキドン酸 / 非ステロイド性消炎鎮痛剤

Outline of Final Research Achievements

Following experiments were performed to clarify the roles of leukotriene B4 receptors BLT1 and BLT2 on inflammation, immunity and barrier function. I succeeded in establishing BLT1 flox/flox mice to generate cell-specific BLT1-deficient mice. We established a monoclonal antibody for mouse BLT1 and identified distinct subsets of macrophages and dendritic cells defined by BLT1 expression. These subsets are different in production of cytokines and lipid mediators. BLT2 was found to protect acute lung injury by a toxin pneumolysin by inhibiting the expression and signaling of cysteinyl leukotrienes. I also clarified the detailed molecular mechanism of BLT2-dependent barrier function in epithelial cells.

Free Research Field

生化学・免疫学