Study on the responsible gene for the diminazene-resistant Babesia gibsoni using the DNA microarray.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07739
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Iwate University
• Principal Investigator: Yamasaki Masahiro 岩手大学, 農学部, 教授 (40322846)
• Co-Investigator(Kenkyū-buntansha): 今井 正樹 東京大学, 医科学研究所, 准教授 (30333363)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: バベシア症 / Babesia gibsoni / ジミナゼン製剤 / 薬剤耐性 / DNAマイクロアレイ / 次世代シークエンサー

Outline of Final Research Achievements

In this study, responsible genes for the diminazene-resistance of Babesia gibsoni, a blood protozoan in dogs, was analyzed. At first, we tried to use the DNA microarray for this investigation. However, there are less reference genes of B. gibsoni for this analysis. Therefore, we tried to use the next generation sequencer, which would not require the reference genes for the analysis. As a result, we obtained over 1,300 sequences, and huge numbers of partial sequence of genes of B. gibsoni.

Free Research Field

獣医内科学