The possible application of Ni2+ for the oral cancer treatment

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nihon University
• Principal Investigator: ASANO Masatake 日本大学, 歯学部, 教授 (10231896)
• Co-Investigator(Kenkyū-buntansha): 尾曲 大輔 日本大学, 歯学部, 助教 (10608699) ; 塩野目 尚 日本大学, 歯学部, 専修医 (10757652) ; 五條堀 孝廣 日本大学, 歯学部, 助教 (10755573)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 塩化ニッケル / 口腔扁平上皮癌 / マトリックスメタロプロテアーゼ / ヌードマウス / NF-kappaB

Outline of Final Research Achievements

In the present study, inhibitory effect of Ni2+ on tumor growth was examined. Human oral squamous cell carcinoma-derived culture cells were injected to nude mice tongue. The tumor mass formation was confirmed 1 week after tumor cell administration. The mice were fed with or without 1 mM NiCl2-containing water for 2 to 3 weeks. The CT images were taken every other week. The cancer mass was observed as a translucent area in control mice. In NiCl2-fed mice, much highly translucent area was observed within the translucent area. Histologically, this area corresponded to the necrotic area in the tumor mass. Using the microarray technique, expressions of various cancer metastasis-related genes were examined. In NiCl2-fed mice, most of these genes were significantly reduced.

Free Research Field

口腔病理学