Antitumor immunities with low dose / low dose rate radiation on murine model with oral squamous cell carcinoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Asahi University
• Principal Investigator: Takayama Eiji 朝日大学, 歯学部, 准教授 (70533446)
• Co-Investigator(Kenkyū-buntansha): 一戸 辰夫 広島大学, 原爆放射線医科学研究所, 教授 (80314219) ; 佐藤 あやの 岡山大学, 自然科学研究科, 准教授 (40303002) ; 増田 潤子 岡山大学, 自然科学研究科, 特任助教 (20424674) ; 近藤 信夫 朝日大学, 歯学部, 教授 (40202072) ; 川木 晴美 朝日大学, 歯学部, 准教授 (70513670) ; 神谷 真子 朝日大学, 経営学部, 准教授 (80181907) ; 梅村 直己 朝日大学, 歯学部, 助教 (80609107) ; 中田 隆博 常葉大学, 健康プロデュース学部, 教授 (40273932) ; 本庶 仁子 広島大学, 原爆放射線医科学研究所, 講師 (80614106) ; 足立 誠 朝日大学, 歯学部, 講師 (10468192)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 腫瘍 / 放射線 / 免疫 / T細胞 / サイトカイン

Outline of Final Research Achievements

Syngeneic mouse subcutaneous and pulmonary tumor models by local subcutaneous and intravenous injection of colon carcinoma CT26 cells were established. Myeloid-derived suppressor cell (MDSC) were significantly increased in the subcutaneous tumor model, but not the pulmonary model. Both CD4+ and CD8+ T cells as well as CD4+ Foxp3+ T cells were significantly decreased in the subcutaneous tumor model, but not the pulmonary model. In addition, the subcutaneous model, but not the pulmonary model, displayed a Th1 polarization bias. This bias was characterized by decreased IL-4,IL-9, and IL-10 production, whereas the pulmonary model displayed increased production of IL-10. These results suggest that the mode of tumor development has differential effects on systemic immunity that may, in turn, influence approaches to treatment of cancer patients.

Free Research Field

口腔生化学