Analysis of mechanism of hypusine pathway in cancer

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Muramatsu Tomoki 東京医科歯科大学, 難治疾患研究所, 助教 (90732553)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: Hypusine経路 / がん転移 / 移動・浸潤 / RNA-CｈIPシークエンス / eIF5A / タンパク質翻訳

Outline of Final Research Achievements

Activation of hypusine pathway, which relates to the translation, contributes to malignant phenotype in cancer. However, since direct target genes of hypusine pathway remain unclear, I tried to identify mRNAs which bind to eIF5A, a translational factor, in this study.
As first, I performed RNA-ChIP assay for isolation of mRNAs, which were binding to eIF5A. After that, I analyzed its sequence using ion proton. Next, I and Dr. Tanimoto, a computational scientist, extracted the candidate genes. To evaluate whether the candidate genes bind to eIF5A, I performed RT-PCR using each specific primer of these genes. Then, I found that some candidate genes bound to eIF5A. In addition, to determine the pathways which are regulated by eIF5A, I performed KEGG pathway analysis against extracted candidate genes. As a result, I found that eIF5A might regulate the translation of cytoskeleton and adhesion molecules. Moreover, we tried to identify the binding motif of eIF5A based on RNA sequencing data.

Free Research Field

腫瘍生物学