2016 Fiscal Year Final Research Report
Development of glycosylated lipid metabolomics system for clarifying their neuronal function
| Project/Area Number |
15K19016
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Fujita Health University (2016)
Institute of Physical and Chemical Research (2015) |
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Principal Investigator |
Nakajima Kazuki 藤田保健衛生大学, その他部局等, 講師 (10442998)
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| Research Collaborator |
Hirabayashi Yoshio 国立研究開発法人理化学研究所, 脳科学総合研究センター・神経膜機能研究チーム, シニアチームリーダー (90106435)
Nagatsuka Yasuko 国立研究開発法人理化学研究所, 脳科学総合研究センター・神経膜機能研究チーム, 研究嘱託
Akiyama Hisako 国立研究開発法人理化学研究所, 脳科学総合研究センター・神経膜機能研究チーム, 研究員 (80623462)
Tohda Koji 富山大学, 大学院理工学研究部(工学), 教授 (60212065)
Iwabuchi Kazuhisa 順天堂大学, 環境医学研究所, 教授 (10184897)
Kamiguchi Hiroyuki 国立研究開発法人理化学研究所, 脳科学総合研究センター・神経成長機構研究チーム, チームリーダー (10233933)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | グルコース化脂質 / 固相抽出 / 親水性相互作用クロマトグラフィー / 気相分離 / 脂質メタボローム / 神経新生 / 肥満 / 腎疾患 |
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| Outline of Final Research Achievements |
Mono-glucosylated sphingolipids and glycerophospholipids play important roles in diverse biologicalprocesses and are linked to a variety of pathologies, such as Parkinson disease.The precise identification of the carbohydrate head group of these lipids is complicated by their isobaric nature and by a low abundant amounts in biological samples. Therefore, the challenging researches for monitoring glucosylated lipids is to develop chromatographic method to separate lipids that are not distinguished by regular MS alone. To overcome these obstacles, we have established a novel target lipidomics workflow for efficiently detecting such lipid species. The workflow includes 1) enrichment by affinity chromatography using newly developed benzoboloxole-modified column, 2) separation by zwitterionic hydrophilic interaction chromatography, 3) gas-phase separation by ion-mobility system, and 4) screening assay system by triple quadrupole mass spectrometer.
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| Free Research Field |
生化学
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