Preparation of IgY antibody for prevention of atopic dermatitis.

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K12712
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Kyoto Women's University
• Principal Investigator: Hatta Hajime 京都女子大学, 家政学部, 教授 (00309056)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: IgY / 黄色ぶどう球菌 / アトピー性皮膚炎 / 増殖抑制

Outline of Final Research Achievements

S. aureus (SA) has been considered one of causative bacteria of atopic dermatitis. The purpose of this study is to evaluate the growth inhibitory effect of anti-SA IgY against S. aureus. This study might lead to a novel application of an anti-SA IgY for prevention of atopic dermatitis using a skin care cream containing an anti-SA IgY antibody. Control IgY or anti-SA IgY was adjusted to a concentration of 0-10 mg/ml, and each was mixed with SA solution at a volume ratio of 1: 1. Bacterial growth was monitored by absorbance at 600 nm or by ATP chemiluminescence assay at 0, 4, 6, 8, 12 and 24 hours incubation at 37 ° C. It was found that the growth inhibitory effect of anti-SA IgY showed delaying the lag phase of SA growth curve for 4 hours compared to that of Control IgY. Four hours of bacteriostatic on SA growth could be a prevention measure against atopic dermatitis in using a skin care cream containing an anti-SA IgY antibody.

Free Research Field

食品科学

Academic Significance and Societal Importance of the Research Achievements

アトピー体質の皮膚常在細菌は表皮ブドウ球菌（SE）より黄色ブドウ球菌(Staphylococcus aureus：SA)が優勢であり、それが産生する毒素が皮膚の炎症を誘発する。本研究では産卵鶏をSA菌体抗原で過免疫し、その鶏卵卵黄から精製した抗 SA IgY抗体（濃度10mg/ml ）がSA菌の増殖誘導期を約４時間遅延させることを示した。このことから、さらに研究が必要であるが、抗SA IgY抗体を配合したクリームを皮膚に４時間毎に塗布することで、SA菌の増殖を抑制しアトピー性皮膚炎の予防に利用できる可能性が期待される。