2017 Fiscal Year Final Research Report
Development of Escherichia coli capable of producing antibody with humanized glycan structure
| Project/Area Number |
16K14886
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大橋 貴生 大阪大学, 生物工学国際交流センター, 助教 (10597876)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 糖鎖 / 大腸菌 / 抗体 |
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| Outline of Final Research Achievements |
Mutant PglB genes were designed based on 10 Campylobacter PglBs, substrate specificity, and 3D structure, and inserted into the expression vector. The transfer activity was examined resulting in decreased activity. Signal sequence from PelB for localization at E. coli periplasmic fraction was linked both H- and L-chains, and expressed in E. col. The expression of H-chain was, even the level was low in soluble fraction, confirmed, but L-chain was not. DsPgl from Desulfovibrio gigaswas picked up instead of PglB. In addition, truncated forms of H- and L-chains were considered to be used to facilitate the project.
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| Free Research Field |
糖鎖生物学
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