Serotonin improves high fat diet induced obesity in mice by the activations of PGC-1 alpha and UCP1 through 5-HTR2A and 7

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15021
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Tohoku University
• Principal Investigator: Aso Hisashi 東北大学, 農学研究科, 教授 (50241625)
• Co-Investigator(Kenkyū-buntansha): 北澤 春樹 東北大学, 農学研究科, 准教授 (10204885) ; 野地 智法 東北大学, 農学研究科, 准教授 (10708001) ; 渡邊 康一 東北大学, 農学研究科, 助教 (80261494)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: セロトニン / 褐色脂肪細胞 / THP1 / エネルギー代謝 / 抗肥満作用

Outline of Final Research Achievements

Mice were fed a chow (Ch) diet or a high fat (F) diet and orally administrated with serotonin (5-HT) every morning between the ages of 5 and 26 weeks. Mice on the F diet gained significantly more weight than the Ch fed mice. In order to identify the target tissues of the effect of 5-HT on energy metabolism, we focused on brawn adipose tissue. PGC-1 is a master regulator that promotes mitochondrial biogenesis and an activity of uncoupling protein (UCP) 1 in brawn adipose tissue. 5-HT increased the expressions of PGC-1-a, b, c and UCP1. Brawn adipose tissue had higher expressions of 5HTR2A, 2B and 7. Mice were pre-treated with several 5-HTR antagonists at 30 min before 5-HT injection. We revealed that 5-HT increased the mRNA expressions of PGC-1and UCP1 through 5-HTR2A and 7.

Free Research Field

細胞生物学