Functional analysis of KCTD protein as a novel angiogenic regulator

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18421
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Ehime University
• Principal Investigator: Sakaue Tomohisa 愛媛大学, 医学系研究科, 助教(特定教員) (20709266)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 血管新生 / CUL3 / ユビキチン / 細胞骨格 / アクチン

Outline of Final Research Achievements

We have recently found CUL3-KCTD complex, one of the E3 ubiquitin ligase, as a novel angiogenic factor. In this study, we screened the substrate of CUL3-KCTD complex using wheat cell-free synthesized protein array, resulting in the successful identification of regulator of GTP-RhoA. Knockdown of CUL3 or KCTD led to severe anti-angiogenic phenotypes due to accumulation of the substrate in endothelial cells. These data suggested that CUL3-KCTD would tightly regulate the substrate protein which might play a crucial role for vascular elongation during angiogenesis.

Free Research Field

血管生物学