Molecular mechanism underlying neuronal polarity

2009 Fiscal Year Final Research Report

• Project/Area Number: 17024024
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: KAIBUCHI Kozo Nagoya University, 大学院・医学系研究科, 教授 (00169377)
• Co-Investigator(Kenkyū-buntansha): NARIKO Arimura 玉川大学, 脳科学研究所, グローバルCOE准教授 (20420375) ; DAISUKE Mori 名古屋大学, 大学院・医学系研究科, COE特任講師 (00381997) ; TOMOKI Nishioka 名古屋大学, 大学院・医学系研究科, 助教 (70435105)
• Project Period (FY): 2005 – 2009
• Keywords: 極性 / 軸索 / シグナル伝達 / CRMP-2 / 小胞体 / 細胞外因子

Research Abstract

Our project aimed at examining the molecular mechanisms underlying establishment and maintenance of neuronal polarity. We found that GSK-3β regulates neuronal polarity through the phosphorylation of CRMP-2. CRMP-2 complex directly links Kinesin-1 motor, and this association is required for the anterograde transport of signaling molecules and polarity related proteins-containing vesicles.

Research Products

• [Journal Article] CRMP-2 directly binds to cytoplasmic dynein and interferes with its activity (2009)
  • Author(s): Arimura, N., Hattori, A., Kimura, T., Nakamuta, S., Funahashi, Y., Hirotsune, S., Furuta, K., Urano, T., Toyoshima, YY.,, Kaibuchi, K.
  • Journal Title: Journal of neurochemistry 111 Pages: 380-90
  • Peer Reviewed
• [Journal Article] Anterograde transport of TrkB in axons is mediated by direct interaction with Slp1 and Rab27 (2009)
  • Author(s): Arimura, N., Kimura, T., Nakamuta, S., Taya, S., Funahashi, Y., Hattori, A., Shimada, A., Mnager, C., Kawabata, S., Fujii, K., Iwamatsu, A., Segal, RA., Fukuda, M.,, Kaibuchi, K.
  • Journal Title: Developmental cell 16 Pages: 675-86
  • Peer Reviewed
• [Journal Article] Neuronal polarity: from extracellular signals to intracellular mechanisms (2007)
  • Author(s): Arimura, N.,, Kaibuchi, K.
  • Journal Title: Nature reviews. Neuroscience 8 Pages: 194-205
  • Peer Reviewed
• [Journal Article] IQGAP3, a novel effector of Rac1 and Cdc42, regulates neurite outgrowth (2007)
  • Author(s): Wang, S., Watanabe, T., Noritake, J., Fukata, M., Yoshimura, T., Itoh, N., Harada, T., Nakagawa, M., Matsuura, Y., Arimura, N.,, Kaibuchi, K.
  • Journal Title: Journal of cell science 120 Pages: 567-77
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of axon specification and neuronal disorders (2006)
  • Author(s): Yoshimura, T., Arimura, N.,, Kaibuchi, K.
  • Journal Title: Annals of the New York Academy of Sciences 1086 Pages: 116-25
  • Peer Reviewed
• [Journal Article] Signaling networks in neuronal polarization (2006)
  • Author(s): Yoshimura, T., Arimura, N.,, Kaibuchi, K.
  • Journal Title: The Journal of neuroscience: the official journal of the Society for Neuroscience 26 Pages: 10626-30
  • Peer Reviewed
• [Journal Article] Ras regulates neuronal polarity via the PI3-kinase/Akt/GSK-3beta/CRMP-2 pathway (2006)
  • Author(s): Yoshimura, T., Arimura, N., Kawano, Y., Kawabata, S., Wang, S.,, Kaibuchi, K.
  • Journal Title: Biochemical and biophysical research communications 340 Pages: 62-8
  • Peer Reviewed
• [Journal Article] Key regulators in neuronal polarity (2005)
  • Author(s): Arimura, N.,, Kaibuchi, K.
  • Journal Title: Neuron 48 Pages: 881-4
  • Peer Reviewed
• [Journal Article] Phosphorylation by Rho kinase regulates CRMP-2 activity in growth cones (2005)
  • Author(s): Arimura, N., Mnager, C., Kawano, Y., Yoshimura, T., Kawabata, S., Hattori, A., Fukata, Y., Amano, M., Goshima, Y., Inagaki, M., Morone, N., Usukura, J., Kaibuchi, K.
  • Journal Title: Molecular and cellular biology 25 Pages: 9973-84
  • Peer Reviewed
• [Journal Article] CRMP-2 is involved in kinesin-1-dependent transport of the Sra-1/WAVE1 complex and axon formation (2005)
  • Author(s): Kawano, Y., Yoshimura, T., Tsuboi, D., Kawabata, S., Kaneko-Kawano, T., Shirataki, H., Takenawa, T.,, Kaibuchi, K.
  • Journal Title: Molecular and cellular biology 25 Pages: 9920-35
  • Peer Reviewed
• [Journal Article] Regulatory machinery of UNC-33 Ce-CRMP localization in neurites during neuronal development in Caenorhabditis elegans (2005)
  • Author(s): Tsuboi, D., Hikita, T., Qadota, H., Amano, M.,, Kaibuchi, K.
  • Journal Title: Journal of neurochemistry 95 Pages: 1629-41
  • Peer Reviewed
• [Journal Article] Tubulin and CRMP-2 complex is transported via Kinesin-1 (2005)
  • Author(s): Kimura, T., Arimura, N., Watanabe, H., Iwamatsu, A.,, Kaibuchi, K.
  • Journal Title: Journal of neurochemistry 93 Pages: 1371-82
  • Peer Reviewed
• [Presentation] Inositol 1, 4, 5-trisphosphate induced Ca2+ release is involved in neurite outgrowth and neuronal polarization (2009)
  • Author(s): 中牟田信一, 船橋靖広, 上口裕之, 貝淵弘三
  • Organizer: 第32回日本神経科学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] Neuronal polarity: From extracellular signals to intracellular mechanisms (2009)
  • Author(s): 貝淵弘三
  • Organizer: 第32回日本神経科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] Axon formation and polarized transport (2009)
  • Author(s): 貝淵弘三
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-22
• [Presentation] Identification of PI 3-kinase as an Slp1-interacting protein in neuron、神経細胞におけるSlp1相互作用タンパク質の同定 (2009)
  • Author(s): 船橋靖広, 有村奈利子, 中牟田信一, 貝淵弘三
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-03
• [Presentation] The role of inositol 1, 4, 5-trisphosphate receptor-induced Ca2+ release in neuronal polarization and neurite outgrowth (2009)
  • Author(s): 中牟田信一, 船橋靖広, 有村奈利子, 上口裕之, 貝淵弘三
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-03
• [Presentation] Axon formation and polarized vesicle transport (2009)
  • Author(s): Kaibuchi, K.
  • Organizer: ESF-EMBO Symposium CELL POLARITY AND MEMBRANE TRAFFIC
  • Place of Presentation: Spain
  • Year and Date: 2009-05-23
• [Presentation] Axon formation and polarized vesicle transport (2008)
  • Author(s): Kaibuchi, K.
  • Organizer: 日本分子生物学会年会日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Identification of Slp1-interacting-proteins in neuron BMB2008 (2008)
  • Author(s): 船橋靖広, 有村奈利子, 吉村武, 中牟田信一, 貝淵弘三
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Axon formation and axonal transport (2008)
  • Author(s): Kaibuchi, K.
  • Organizer: 日本神経化学会シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2008-09-11
• [Presentation] Neuronal polarity and small (2008)
  • Author(s): Kaibuchi, K.
  • Organizer: GTPases FASEB summer research conferences
  • Place of Presentation: Vermont, USA
  • Year and Date: 2008-07-16
• [Presentation] Neuronal polarity and axonal transport (2008)
  • Author(s): Kaibuchi, K.
  • Organizer: 日本神経科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-08
• [Presentation] Neuronal polarity and axonal transport (2008)
  • Author(s): Kaibuchi, K.
  • Organizer: Gordon research conference
  • Place of Presentation: Hong Kong, China
  • Year and Date: 2008-06-11
• [Presentation] Identification of ULK1 as a novel CRMP-2 phosphorylation kinase (2007)
  • Author(s): 島田明子, 有村奈利子, 貝淵弘三
  • Organizer: BMB2007、第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] CRMP-2 and GSK-3beta regulate anterograde TrkB transport through Slp1 (2007)
  • Author(s): Arimura N.,, Kaibuchi, K.
  • Organizer: ASCB 47th Annual meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2007-12-01
• [Presentation] CRMP-2, Slp1 and Rab27B mediate anterograde transport of TrkB (2007)
  • Author(s): 有村奈利子, 島田明子, 貝淵弘三
  • Organizer: Neuro2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Presentation] Rho-kinaseおよびGSK-3beta阻害剤による軸索伸長制御のメカニズム (2007)
  • Author(s): 有村奈利子, 吉村武, 貝淵弘三
  • Organizer: 第18回日本末梢神経学会
  • Place of Presentation: 弘前
  • Year and Date: 2007-08-24
• [Presentation] CRMP-2 and Slp1 mediate anterograde transport of TrkB through Kinesin-1 (2006)
  • Author(s): Arimura N.,, Kaibuchi, K.
  • Organizer: 46th annual meeting of American Society of Cell Biology
  • Place of Presentation: San Diego. USA
  • Year and Date: 2006-12-09
• [Presentation] Ubiquitination of collapsin response mediator protein-2 participates in neuronal polarization (2006)
  • Author(s): 李隼庸, 有村奈利子, 貝淵弘三
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-06
• [Presentation] The role of CRMP-2 as a novel cytoplasmic dynein interacting molecule (2006)
  • Author(s): 朋部敦志, 有村奈利子, 貝淵弘三
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-06
• [Presentation] Dynamics of signaling molecules during axon specification (2006)
  • Author(s): Yoshimura, T.,, Kaibuchi, K.
  • Organizer: 36th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Atlanta. USA
  • Year and Date: 2006-11-14
• [Presentation] Cytoskeletal Dynamics and Signal Transduction in Neuronal Polarity (2006)
  • Author(s): Kaibuchi, K.
  • Organizer: 36th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Atlanta
  • Year and Date: 2006-10-14
• [Presentation] Signaling for neuronal polarization (2006)
  • Author(s): Kaibuchi, K.
  • Organizer: 36th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Atlanta. USA
  • Year and Date: 2006-10-14
• [Presentation] Role of Slp, a novel CRMP-2 interacting molecule, in hippocampal neurons (2006)
  • Author(s): 吉村武, 有村奈利子, 貝淵弘三
  • Organizer: 第49回日本神経化学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-14
• [Presentation] Signaling for neuronal polarization (2006)
  • Author(s): 貝淵弘三
  • Organizer: Neuroscience 2006
  • Place of Presentation: 名古屋
  • Year and Date: 2006-07-19
• [Presentation] 新規Collapsin response mediator protein-2結合蛋白質synaptotagmin-like proteinの海馬神経細胞における機能解析 (2006)
  • Author(s): 有村奈利子, 川端紗枝子, 朋部敦志, 貝淵弘三
  • Organizer: 第29回日本神経科学大会
  • Place of Presentation: 京都
  • Year and Date: 2006-06-19
• [Presentation] Key regulators in neuronal polarization (2006)
  • Author(s): Kaibuchi, K.,, Arimura N.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
• [Presentation] Ras Regulates Neuronal Polarity via PI3-kinase/Akt/GSK-3β/CRMP-2 pathway (2005)
  • Author(s): Kaibuchi, K.
  • Organizer: The American Society For Cell Biology 45th Annual Meeting
  • Place of Presentation: San Francisco. USA
  • Year and Date: 2005-12-10
• [Presentation] Neuronal polarity and cytoskeleton (2005)
  • Author(s): Yoshimura, T.,, Kaibuchi, K.
  • Organizer: The American Society For Cell Biology 45th Annual Meeting
  • Place of Presentation: San Francisco. USA
  • Year and Date: 2005-12-10
• [Presentation] 細胞の極性形成と遊走を制御する分子機構の解明 (2005)
  • Author(s): 貝淵弘三
  • Organizer: 第28回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-07
• [Presentation] 伝達分子と軸索伸長制御の分子メカニズムシグナル (2005)
  • Author(s): 貝淵弘三
  • Organizer: 伝達分子と軸索伸長制御の分子メカニズムシグナル
  • Place of Presentation: 岡崎
  • Year and Date: 2005-11-24
• [Presentation] Microtubule dynamics and cell polarity (2005)
  • Author(s): Kaibuchi, K.
  • Organizer: 9th International Dahlem Symposium on Cellular Signal Recognition and Transduction
  • Place of Presentation: Berlin
  • Year and Date: 2005-10-13
• [Presentation] Neuronal polarity and axon formation (2005)
  • Author(s): 貝淵弘三
  • Organizer: 第48回日本神経化学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-09-28
• [Presentation] IQGAP3, a novel effector of Rac1 and Cdc42, regulates neurite outgrowth through actin cytoskeleton reorganization (2005)
  • Author(s): 王淑杰, 渡辺崇, 則武淳, 佐藤和正, 吉村武, 深田正紀, 貝淵弘三
  • Organizer: 第48回日本神経化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2005-09-28
• [Presentation] Ras regulates neuronal polarity via the PI3-kinase/Akt/GSK-3β/CRMP-2 pathway (2005)
  • Author(s): 吉村武, 貝淵弘三
  • Organizer: 第48回日本神経化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2005-09-28
• [Presentation] Role of CRMP-2 in the establishment of neuronal polarity (2005)
  • Author(s): Kaibuchi, K.
  • Organizer: 20th Biennial Meeting of the ISN
  • Place of Presentation: Innsbruck
  • Year and Date: 2005-08-21
• [Presentation] 神経細胞の極性形成におけるPar複合体の役割 (2005)
  • Author(s): 西村隆史, 貝淵弘三
  • Organizer: 第28回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2005-07-26
• [Presentation] 神経細胞の極性形成機構 (2005)
  • Author(s): 貝淵弘三
  • Organizer: 日本分子生物学会第5回春期シンポジウム
  • Place of Presentation: 新潟
  • Year and Date: 2005-05-15
• [Presentation] Signaling for Neuronal Polarity (2005)
  • Author(s): Kaibuchi, K.
  • Organizer: Keystone Symposia on Cell Migration and Adhesion
  • Place of Presentation: Snowbird, Utah
  • Year and Date: 2005-04-09
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/Yakuri/