Genetic analysis of polyamine production mechanism in the intestinal lumen of the dominant human intestinal bacteria

2019 Fiscal Year Final Research Report

• Project/Area Number: 17H05026
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Kindai University (2019); Ishikawa Prefectural University (2017-2018)
• Principal Investigator: Kurihara Shin 近畿大学, 生物理工学部, 講師 (20630966)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: ポリアミン / ヒト腸内常在菌叢最優勢種 / Bacteroides dorei / B. thetaiotaomicron / Enterococcus faecalis / 腸内細菌の培養 / 腸内細菌の遺伝子操作

Outline of Final Research Achievements

A comprehensive analysis of polyamine synthesis and transport in the most dominant species of the human intestinal microbiota and Bifidobacteria, which are typical beneficial bacteria, was performed. Then, genetic and biochemical analysis of polyamine metabolism and transport systems in a single bacterium or spanning multiple bacteria in the phylum Bacteroidetes and Firmicutes, the two most dominant phylum in the human intestinal microbiota, was carried out. Furthermore, two novel polyamine exporters in P. mirabilis, an intestinal bacterium that releases significant amounts of polyamines into the environment, have been screened. These results will provide important basic scientific insights for optimizing polyamine concentrations in the intestinal lumen.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

近年、世界中の研究者が生体内のポリアミン量を上昇させると動物の健康寿命が伸長することを報告しており、ポリアミン摂取による医療費削減・労働人口増大が期待されている。腸内細菌はヒトの主要なポリアミン源であるが、その腸管内腔での生産機構はほとんど明らかとなっていない。本研究で得られた成果は腸管内腔におけるポリアミン濃度を最適化するための重要な基礎科学的知見となる。