2022 Fiscal Year Final Research Report

Classifing undifferentiated/unclassified sarcomas by focusing on the Wnt signaling pathway

Research Project

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Project/Area Number	17K08748
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Human pathology
Research Institution	Kanazawa Medical University
Principal Investigator	KOHNO Hiroyuki 金沢医科大学, 看護学部, 教授 (20221236)
Co-Investigator(Kenkyū-buntansha)	尾山武金沢大学, 医学系, 助教 (00515314)
Project Period (FY)	2017-04-01 – 2023-03-31
Keywords	未分化/未分類肉腫 / Array CGH / FISH / Wnt pathway / b-catenin / 免疫組織化学
Outline of Final Research Achievements	Undifferentiated/unclassified sarcomas, which occur infrequently and present a variety of histologic types, lack highly specific markers, and the search for new specific markers is important for the diagnosis of these tumors. We collected information on undifferentiated pleomorphic sarcoma/undifferentiated pleomorphic sarcoma/malignant fibrous histiocytoma archived in the pathology department of our hospital, with key clinical information such as the presence or absence of metastases. We focused on STAT3 as a candidate marker gene for differentiated/unclassified sarcomas. RP11-341F8 was selected as its Bacterial Artificial clone, from which BAC DNA was amplified and fluorescent labeled to create a FISH probe. FISH was performed on undifferentiated/unclassified sarcomas, and no STAT3 gene amplification was observed.
Free Research Field	病理学
Academic Significance and Societal Importance of the Research Achievements	未分化/未分類肉腫に分類される悪性軟部腫瘍は、頻度が低く特異性の高いマーカーも無いため、しばしば病理診断に難渋し病理医間でも診断が分かれることが多く、現時点においても分類のカテゴリーが変更になる可能性もあり得る。このような未分化/未分類肉腫に分類される悪性軟部腫瘍を診断する際の補助として、特異性の高いマーカーが求められている。本研究では、候補遺伝子としてSTAT３に着目し、遺伝子増幅を検索したが、増幅は確認できなかった。STAT3遺伝子は、遺伝子再構成やDNAメチル化によってその機能を変化させている可能性も考えられるので、今後は次世代シーケンサーやメチローム解析を含めた解析を予定している。