2018 Fiscal Year Final Research Report
Novel biomarker of bile duct cancer using metabolome analysis of bile juice
Project/Area Number |
17K15975
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Osaka Medical College |
Principal Investigator |
Ogura Takeshi 大阪医科大学, 医学部, 講師 (10633331)
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Project Period (FY) |
2017-04-01 – 2019-03-31
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Keywords | メタボローム解析 |
Outline of Final Research Achievements |
50 uL of plasma (or serum) were added to 450 uL 181;L of methanol containing internal standards (Solution ID: H3304-1002, Human Metabolome Technologies, Inc., Tsuruoka, Japan) at 0℃186;C in order to inactivate enzymes. The extract solution was thoroughly mixed with 500 uL 181;L of chloroform and 200 uL 181;L of Milli-Q water and centrifuged at 2,300 ×g and 4uL 186;C for 5 min. The 350 uL 81;L of upper aqueous layer was centrifugally filtered through a Millipore 5-kDa cutoff filter to remove proteins. The filtrate was centrifugally concentrated and re-suspended in 50 uL 181;L of Milli-Q water for CE-MS analysis. Finally, A was significant factor associated with malignancy.
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Free Research Field |
消化器病学
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Academic Significance and Societal Importance of the Research Achievements |
物質Aが同定された。本結果は、胆道癌の診断の際、胆汁細胞診を行うが、感度が低いこと、偽陽性が多いことが問題であった。しかし、胆汁中の物質Aを測定することで癌の補助診断に有用である可能性が示唆された。さらに前向き試験として行い、感度、特異度を算出することで、より確立された手法になる可能性が示唆される。
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