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2010 Fiscal Year Final Research Report

Field trial of transgenic poplars for industrial use

Research Project

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Project/Area Number 19208016
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産科学・木質工学
Research InstitutionTokyo University of Agriculture (2009-2010)
Kyoto University (2007-2008)

Principal Investigator

HAYASHI Takahisa  東京農業大学, 応用生物科学部, 教授 (70231529)

Co-Investigator(Kenkyū-buntansha) 谷口 亨  森林総合研究所, 森林バイオ研究センター, 研究員 (00360470)
馬場 啓一  京都大学, 生存圏研究所, 助教 (20238223)
海田 るみ  東京農業大学, 応用生物科学部, 研究員 (00398885)
太田 誠一  京都大学, 大学院・農学研究科, 教授 (10346033)
磯貝 明  東京大学, 大学院・農学生命研究科, 教授 (40191879)
舘野 正樹  東京大学, 理学研究科, 准教授 (00179730)
吉田 正人  名古屋大学, 生命農学研究科, 准教授 (30242845)
富田 裕子 (半場 裕子)  京都工芸繊維大学, 学内共同利用センター, 准教授 (90314666)
古田 裕三  京都府立大学, 生命環境科学科, 准教授 (60343406)
菊池 彰  筑波大学, 大学院・生命環境科学研究科, 講師 (00400648)
西窪 伸之  独立行政法人理化学研究所, 植物科学センター, 研究員 (30392062)
荒川 圭太  北海道大学, 大学院・農学研究科, 准教授 (00241381)
加来 友美  京都大学, 生存圏研究所, 研究員 (10378911)
朴 龍又  京都大学, 生存圏研究所, 研究員 (00378910)
栗田 学  独立行政法人林木育種センター, 研究員 (40370829)
坪村 美代子  独立行政法人林木育種センター, 研究員 (70415040)
渡邊 和男  筑波大学, 大学院・生命環境科学研究科, 教授 (90291806)
間野 絵梨子  京都大学, 生存圏研究所, 研究員 (60447935)
Project Period (FY) 2007 – 2010
Keywords組織構造 / 材形成
Research Abstract

The transgenic expression of Aspergillus xyloglucanase cDNA(AaXEG2) with 35S promoter in the leaves of open-field grown poplars was studied. This level was slightly decreased in the poplars grown in fertile soil for four years. Xyloglucan content was greatly decreased in the walls of the tissues in the transgenic lines, although the degradation levels of xyloglucan were slightly lower for the poplars grown in fertile soil than for those grown in non-fertile soil. The leaves exhibited a smaller surface area with more rounded teeth than did those of the wild type plants, as if they were of the sun leaf variety that was grown in the incubation room and subsequently greenhoused. Large numbers of proteins were markedly downregulated in the leaves of the transgenic plants via proteomic analysis. Significant reduction occurred not only on the moduli of elasticity and rupture in both their live stems and dried trunks compared with those in the wild type.

  • Research Products

    (45 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (36 results) (of which Peer Reviewed: 33 results) Presentation (7 results) Book (2 results)

  • [Journal Article] Hemicelluloses as recalcitrant components for saccharification in wood, Buckeridge MS and Goldman GH(eds.)2011

    • Author(s)
      Hayashi T, Kaida R
    • Journal Title

      Routes to Cellulosic Ethanol, Springer Science

      Pages: 45-52

    • Peer Reviewed
  • [Journal Article] Overexpression of xyloglucanase(AaXEG2) accelerates heteroblastic development in mangium leaves2011

    • Author(s)
      Hartati S, Sudarmonowati E, Kaku T, Ikegaya H, Baba K, Kaida R, Hayashi T
    • Journal Title

      J Wood Sci

      Volume: 57 Pages: 463-469

    • Peer Reviewed
  • [Journal Article] Improvement of fermentable sugar yields of mangium through transgenic overexpression of xyloglucanase2011

    • Author(s)
      Kaku T, Kaida R, Baba K, Hartati S, Sudarmonowati E, Hayashi T
    • Journal Title

      J Wood Sci

      Volume: 57 Pages: 545-548

    • Peer Reviewed
  • [Journal Article] Enzymatic modification of plant cell wall polysaccharides2011

    • Author(s)
      Obro J, Hayashi T, Mikkelsen JD
    • Journal Title

      Annual Plant Reviews

      Volume: 41 Pages: 367-388

    • Peer Reviewed
  • [Journal Article] Meranti bakau as a potential wood species in Riau Peat Swamp Forest2011

    • Author(s)
      Dwianto W, Fitria, Gunawan H, Hayashi T
    • Journal Title

      In Sustainable Management of Bio-resources in Tropical Peat-swamp Forest

      Pages: 25-41

    • Peer Reviewed
  • [Journal Article] Plant fiber formation : state of the art, recent and expected progress and open questions2011

    • Author(s)
      Gorshkova T, Brutch N, Chabbert B, Deyholos M, Hayashi T, Lev-Yadun S, Mellerowicz EJ, Morvan C, Neutelings G, Pilate G
    • Journal Title

      Critical Reviews in Plant Sciences

      Volume: 31 Pages: 201-228

    • Peer Reviewed
  • [Journal Article] Functions of xyloglucan in plant cells2011

    • Author(s)
      Hayashi T, Kaida R
    • Journal Title

      Mol Plant

      Volume: 4 Pages: 17-24

    • Peer Reviewed
  • [Journal Article] Wood characteristic of superior sengon(Paraserianthes falcataria) collection and prospect of wood properties improvement through genetic engineering2010

    • Author(s)
      Hartati NS, Sudarmonowati E, Fatriasari W, Hermiati E, Dwianto W, Kaida R, Baba K, Hayashi T
    • Journal Title

      Wood Res J

      Volume: 1 Pages: 104-108

    • Peer Reviewed
  • [Journal Article] Enlargement of individual cellulose microfibril in transgenic poplars overexpressing xyloglucanase2010

    • Author(s)
      Yamamoto R, Saito T, Isogai A, Kurita M, Kondo T, Taniguchi T, Kaida R, Baba K, Hayashi T
    • Journal Title

      J Wood Sci

      Volume: 57 Pages: 71-75

    • Peer Reviewed
  • [Journal Article] Enhancing primary raw materials for biofuels2010

    • Author(s)
      Hayashi T, Kaida R, Mistuda N, Ohme-Takagi M, Nishikubo N, Kidou S, Yoshida K
    • Journal Title

      Biomass to Biofuels

      Pages: 459-489

    • Peer Reviewed
  • [Journal Article] Loosening xyloglucan prevents tensile stress in tree stem bending but accelerates the enzymatic degradation of cellulose2010

    • Author(s)
      Hayashi T, Kaida R, Kaku T, Baba K
    • Journal Title

      Russian J Plant Physiol

      Volume: 57 Pages: 316-320

    • Peer Reviewed
  • [Journal Article] Potential role for purple acid phosphatase in the dephosphorylation of wall proteins in tobacco cells2010

    • Author(s)
      Kaida, R, Serada, S, Norioka, N, Norioka, S, Neumetzler, L, Pauly, M, Sampedro, J, Zarra, I, Hayashi, T, Kaneko, TS
    • Journal Title

      Plant Physiol

      Volume: 153 Pages: 603-610

    • Peer Reviewed
  • [Journal Article] Acceleration of cell growth by xyloglucan oligosaccharides in suspension-cultured tobacco cells2010

    • Author(s)
      Kaida R, Sugawara S, Negoro K, Maki H, Hayashi T, Kaneko TS
    • Journal Title

      Mol Plant

      Volume: 3 Pages: 549-554

    • Peer Reviewed
  • [Journal Article] Sucrose synthase is an integral component of the cellulose synthesis machinery2010

    • Author(s)
      Fujii S, Hayashi T, Mizuno K
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Pages: 294-301

    • Peer Reviewed
  • [Journal Article] Plasticity of xyloglucan composition in bean(Phaseolus vulgaris)-cultured cells during habituation and dehabituation to lethal concentrations of dichlobenil2010

    • Author(s)
      Alonso-Simon A, Neumetzler L, Garcia-Angulo P, Encina AE, Acebes JL, Alvarez JM, Hayashi T
    • Journal Title

      Mol Plant

      Volume: 3 Pages: 603-609

    • Peer Reviewed
  • [Journal Article] Proteomic analysis of the G-layer in poplar tension wood2009

    • Author(s)
      Kaku T, Serada S, Baba K, Tanaka F, Hayashi T
    • Journal Title

      J. Wood Sci

      Volume: 55 Pages: 250-257

    • Peer Reviewed
  • [Journal Article] Activation ofβ-glucan synthases by wall-bound purple acid phosphatase in tobacco cells2009

    • Author(s)
      Kaida R, Satoh Y, Bulone V, Yamada Y, Kaku T, Hayashi T, Kaneko T
    • Journal Title

      Plant Physiol

      Volume: 150 Pages: 1822-1830

    • Peer Reviewed
  • [Journal Article] Xyloglucan for generating tensile stress to bend tree stem2009

    • Author(s)
      Baba K, Park WP, Kaku T, Kaida R, Takeuchi M, Yoshida M, Hosoo Y, Ojio Y, Okuyama T, Taniguchi T, Ohmiya Y, Kondo T, Shani J, Shoseyov O, Awano T, Serada S, Norioka N, Norioka S, Hayashi T
    • Journal Title

      Mol Plant

      Volume: 2 Pages: 893-903

    • Peer Reviewed
  • [Journal Article] Loosening xyloglucan accelerates the enzymatic degradation of cellulose in wood2009

    • Author(s)
      Kaida R, Kaku T, Baba K, Oyadomari M, Watanabe T, Nishida K, Kanaya T, Shani Z, Shoseyov O, Hayashi T
    • Journal Title

      Mol Plant

      Volume: 2 Pages: 904-909

    • Peer Reviewed
  • [Journal Article] Enhancement of saccharification by overexpression of poplar cellulase in sengon2009

    • Author(s)
      Kaida R, Kaku T, Baba K, Hartati S, Sudarmonowati E, Hayashi T
    • Journal Title

      J Wood Sci

      Volume: 55 Pages: 435-440

    • Peer Reviewed
  • [Journal Article] Enzymatic saccharification and ethanol production of Acacia mangium and Paraserianthes falcataria wood, and oil palm trunk2009

    • Author(s)
      Kaida R, Kaku T, Baba K, Hayashi T
    • Journal Title

      J Wood Sci

      Volume: 55 Pages: 381-386

    • Peer Reviewed
  • [Journal Article] Enrichment of sugar content in melon fruits by hydrogen peroxide treatment2009

    • Author(s)
      K Ozaki, A Uchida, T Takabe, F Shinagawa, Y Tanaka, T Takabe, T Hayashi, T Hattori, AK. Raid, T Takabe
    • Journal Title

      J Plant Physiol

      Volume: 166 Pages: 569-578

    • Peer Reviewed
  • [Journal Article] Cross-linking of plant cell walls with dehydrated fructose by smoke-heat treatment2008

    • Author(s)
      T Hayashi, YW Park, A Isogai and T Nomura
    • Journal Title

      J Wood Sci

      Volume: 54 Pages: 90-93

    • Peer Reviewed
  • [Journal Article] Biosafety assessment of transgenic poplars overexpressing xyloglucanase(AaXEG2) prior to field trials2008

    • Author(s)
      T Taniguchi, Y Ohmiya, M, Kurita, M Tsubomura, T Kondo, YW Park, K Baba, T Hayashi
    • Journal Title

      J Wood Sci

      Volume: 54 Pages: 408-413

    • Peer Reviewed
  • [Journal Article] Purple acid phosphatase in the walls of tobacco cells2008

    • Author(s)
      R Kaida, T Hayashi, TS Kaneko
    • Journal Title

      Phytochem

      Volume: 69 Pages: 2546-2551

    • Peer Reviewed
  • [Journal Article] Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants2008

    • Author(s)
      T Takabe T, A Uchida A, F Shinagawa, Y Terada, H Kajita, Y Tanaka, T Takabe, T Hayashi, T Kawai, T Takabe
    • Journal Title

      Plant Growth Reg

      Volume: 56 Pages: 265-273

    • Peer Reviewed
  • [Journal Article] Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon2008

    • Author(s)
      S Hartati, E Sudarmonowati E, YW Park YW, T Kaku, R Kaida, K Baba, T Hayashi
    • Journal Title

      Plant Physiol

      Volume: 147 Pages: 552-561

    • Peer Reviewed
  • [Journal Article] Enhancement of saccharification by overexpression of various endoglycanase in poplar2008

    • Author(s)
      T Hayashi, R Kaida, T Kaku, K Baba
    • Journal Title

      J Brasil Sci

      Volume: 55 Pages: 145-149

    • Peer Reviewed
  • [Journal Article] Xyloglucan : The molecular muscle of trees2008

    • Author(s)
      EJ Mellerowicz, P Immerzeel, T Hayashi
    • Journal Title

      Annals Bot

      Volume: 102 Pages: 659-665

    • Peer Reviewed
  • [Journal Article] 遺伝子組換え樹木の野外試験-キシログルカナーゼの過剰発現による高セルロース含量ポプラ-2008

    • Author(s)
      谷口亨、林隆久
    • Journal Title

      BRAINテクノニュース

      Volume: 130 Pages: 22-26

  • [Journal Article] 高セルロース含量ギンドロ(Populus alba L.)の隔離ほ場栽培試験2008

    • Author(s)
      谷口亨
    • Journal Title

      北海道の林木育種

      Volume: Vol.51No.1 Pages: 32-34

  • [Journal Article] 遺伝子組換えポプラの隔離ほ場試験の開始2007

    • Author(s)
      谷口亨
    • Journal Title

      林木の育種

      Volume: 224 Pages: 31-33

  • [Journal Article] Increase in XET activity in bean(Phaseolus vulgaris L.) cells habituated to dichlobenil2007

    • Author(s)
      A Alonso-Simon, P Garcia-Angulo, AE Encina, JM Alvarez, JL Acebes and T Hayashi
    • Journal Title

      Planta

      Volume: 226 Pages: 765-771

    • Peer Reviewed
  • [Journal Article] Xyloglucan Endo-transglycosylase(XET) Functions in Gelatinous Layers of Tension Wood Fibers in Poplar. A Glimpse into the Mechanism of the Balancing Act of Trees2007

    • Author(s)
      N Nishikubo, T Awano, A Banasiak, V Bourquin, F Ibatullin, R Funada, H Brumer, TT Teeri, T Hayashi, B Sundberg and EJ Mellerowicz
    • Journal Title

      Plant Cell Physiol

      Volume: 48 Pages: 843-855

    • Peer Reviewed
  • [Journal Article] Structural organization and a standardized nomenclature for plant endo-1, 4-β-glucanases(cellulases) of glycosyl hydrolase family 92007

    • Author(s)
      BR Urbanowicz, AB Bennett, E del Campillo, C Catala, T Hayashi, B Henrissat, H Hofte, SJ McQueen-Mason, SE Patterson, O Shoseyov, TT Teeri and JKC Rose
    • Journal Title

      Plant Physiology

      Volume: 144 Pages: 1693-1696

    • Peer Reviewed
  • [Journal Article] Analyses of leaves from open field-grown transgenic poplars overexpressing xyloglucanase

    • Author(s)
      Kaku T, Baba K, Taniguchi T, Kurita M, Konagaya K, Ishii K, Kondo T, Serada S, Iizuka H, Kaida R, Taji T, Sakata Y, Hayashi T
    • Journal Title

      J Wood Sci

    • DOI

      DOI:10.1007/s10086-011-1247-1・2012

    • Peer Reviewed
  • [Presentation] キシログルカナーゼを過剰発現させた組換えポプラの野外での成長と根萌芽の発生について2011

    • Author(s)
      谷口亨・栗田学・小長谷賢一・石井克明・舟橋史晃・太田誠一・馬場啓一・加来友美・海田るみ・林隆久
    • Organizer
      第122回森林学会大会
    • Place of Presentation
      静岡大学
    • Year and Date
      20110325-28
  • [Presentation] キシログルカナーゼ過剰発現ポプラの隔離ほ場栽培試験2010

    • Author(s)
      谷口亨、栗田学、近藤禎二、石井克明、太田誠一、馬場啓一、林隆久
    • Organizer
      第28回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      20100902-03
  • [Presentation] Occurrence of xyloglucan in the cellulose microfibrils of the secondary wall2010

    • Author(s)
      Hayashi T and Kaida R
    • Organizer
      XII Cell Wall Meeting
    • Place of Presentation
      Porto, Portogal
    • Year and Date
      2010-07-27
  • [Presentation] キシログルカナーゼ過剰発現ポプラの食葉性昆虫に対する摂食試験2008

    • Author(s)
      谷口亨、栗田学、林隆久、馬場啓一、近藤禎二
    • Organizer
      第26回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      20080901-02
  • [Presentation] さまざまな細胞壁分解酵素を発現する組換えポプラ2008

    • Author(s)
      林隆久、馬場啓一
    • Organizer
      理研シンポジウム
    • Place of Presentation
      理研横浜
    • Year and Date
      2008-02-18
  • [Presentation] Xyloglucan creates tensile stress in the secondary wall2007

    • Author(s)
      T. Hayashi, M. Takeuchi, Y. W. Park, T. Kaku, M. Yoshida, T. Awano, R. Kaida, K. Baba
    • Organizer
      XI Cell Wall Meeting 15^<th>
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      20070800
  • [Presentation] Current situation of researches on genetically modified trees in Japan2007

    • Author(s)
      林隆久
    • Organizer
      フィーラム:少資源国日本のバイオマス研究、日本分子生物学会生化学会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-14
  • [Book] 森をとりもどすために22010

    • Author(s)
      林隆久
    • Total Pages
      171
    • Publisher
      海青社
  • [Book] 森をとりもどすために2008

    • Author(s)
      林隆久
    • Publisher
      海青社

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Published: 2013-07-31   Modified: 2016-01-20  

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