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2009 Fiscal Year Final Research Report

Biological functions of amelogenin's splicing isoforms onchondrocyte differentiation and cartirage formation.

Research Project

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Project/Area Number 19390527
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTohoku University

Principal Investigator

SAEKI Syuichi  Tohoku University, 大学院・歯学研究科, 大学院非常勤講師 (60271954)

Co-Investigator(Kenkyū-buntansha) HARUYAMA Naoto  東京医科歯科大学, 歯と骨のGCOE拠点, GCOE拠点形成特任教員 (70359529)
IGARASHI Kaoru  東北大学, 大学院・歯学研究科, 教授 (70202851)
Project Period (FY) 2007 – 2009
Keywords歯科矯正学 / アメロジェニン / エナメルタンパク / スプライスアイソフォーム
Research Abstract

Amelogenin is a major protein expressed in enamel tissue and playing an important role for enamel formation. However, recent studies show that the amelogenin is expressed in various tissues including cartilage. Especially, two amelogenins among a number of splice isoforms, a full length amelogenin (M180) and a leucine rich amelogenin peptide (LRAP), are implicated as signaling molecules in the mesenchymal cells. In this study, we examined the effects of M180 and LRAP on chondrogenesis. Using differentiation medium supplemented with various concentrations of the M180 or LRAP containing medium, ATDC5 cells, a chondrogenic cell line, were cultivated. Addition of a higher concentration of M180 or LRAP to ATDC5 cells increased the alkaline phosphatase (ALP) activity at 14 days of culture compared to that in control. Increased glycosaminoglycan (GAG) secretion was also observed by alcian blue staining at 21 days of culture. Gene expression levels of ALP, Aggrecan, Col10a1 and Osteopontin were significantly increased in the samples supplemented with amelogenin at 28 days of culture. M180 and LRAP may have significant role to accelerate the chondrogenic differentiation.

  • Research Products

    (20 results)

All 2010 2009 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (12 results) Book (2 results)

  • [Journal Article] Osteogenesis by gradually expanding the interface between bone surface and periosteum enhanced by bone marrow stem cell administration in Rabbits2010

    • Author(s)
      Sato K, Haruyama N, Shimizu Y, Hara J, Kawamura H.
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol Endod

    • URL

      http://dx.doi.org/10.1016/j.tripleo.2009.11.005

    • Peer Reviewed
  • [Journal Article] Genetic evidence for key roles of decorin and biglycan in dentin mineralization2009

    • Author(s)
      Haruyama N, Sreenath TL, Suzuki S, Yao X, Wang Z, Wang Y, Honeycutt C, Iozzo RV, Young MF, Kulkarni AB.
    • Journal Title

      Matrix Biol. 28(3)

      Pages: 129-136

    • URL

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2683192/

    • Peer Reviewed
  • [Journal Article] Synergistic Roles of Amelogenin and Ameloblastin2009

    • Author(s)
      Hatakeyama J, Fukumoto S, Nakamura T, Haruyama N, Suzuki S, Hatakeyama Y, Shum L, Gibson CW, Yamada Y, Kulkarni AB.
    • Journal Title

      J Dent Res. 88(4)

      Pages: 318-322

    • URL

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2758615/

    • Peer Reviewed
  • [Journal Article] In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development2009

    • Author(s)
      Choi SJ, Roodman GD, Feng JQ, Song IS, Amin K, Hart PS, Wright JT, Haruyama N, Hart TC.
    • Journal Title

      Dev Biol. 325(1)

      Pages: 129-137

    • URL

      http://dx.doi.org/10.1016/j.ydbio.2008.10.014

    • Peer Reviewed
  • [Journal Article] Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization2009

    • Author(s)
      Suzuki S, Sreenath T, Haruyama N, Honeycutt C, Terse A, Cho A, Kohler T, Muller R, Goldberg M, Kulkarni AB.
    • Journal Title

      Matrix Biol. 28(4)

      Pages: 221-229

    • URL

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2758621/

    • Peer Reviewed
  • [Journal Article] DSPP effects on in vivo bone mineralization2008

    • Author(s)
      Verdelis K, Ling Y, Sreenath T, Haruyama N, Macdougall M, van der Meulen MC, Lukashova L, Spevak L, Kulkarni AB, Boskey AL.
    • Journal Title

      Bone 43(6)

      Pages: 983-990

    • URL

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2621360/

    • Peer Reviewed
  • [Presentation] 骨膜延長による骨誘導に対する骨髄由来間葉系幹細胞の有効性の検討2010

    • Author(s)
      佐藤光一郎, 春山直人, 清水良央, 原純一, 川村仁
    • Organizer
      第9回日本再生医療学会総会
    • Place of Presentation
      広島
    • Year and Date
      20100318-20100319
  • [Presentation] Amelogenin splice isoforms stimulate chondrogenic differentiation of ATDC5 cells2010

    • Author(s)
      Mitani K, Haruyama N, Igarashi K.
    • Organizer
      The 39^<th> Annual Meeting & Exhibition of the AADR/CADR
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      20100303-20100306
  • [Presentation] 筋ジストロフィーマウスにおける筋機能活性化が下顎骨の成長に与える影響2009

    • Author(s)
      三戸天元, 春山直人, 五十嵐薫
    • Organizer
      第68回日本矯正歯科学会
    • Place of Presentation
      福岡
    • Year and Date
      20091116-20091118
  • [Presentation] DSPPの象牙質石灰化における役割~マウスモデルの表現型解析から~2009

    • Author(s)
      春山直人
    • Organizer
      第2回 顎顔面の器官発生・形態形成研究会
    • Place of Presentation
      軽井沢
    • Year and Date
      20091017-20091018
  • [Presentation] 骨-骨膜延長による骨誘導に対する骨髄由来間葉系幹細胞の有効性の検討2009

    • Author(s)
      佐藤光一郎, 春山直人, 清水良央, 原純一, 川村仁
    • Organizer
      第54回社団法人日本口腔外科学会総会・学術大会
    • Place of Presentation
      札幌
    • Year and Date
      20091009-20091011
  • [Presentation] Osteogenesis by gradually expanding the interface between bone surface and periosteum: Preliminary analysis of the use of novel plate and bone marrow stem cell administration in rabbit2009

    • Author(s)
      Sato K, Haruyama N, Shimizu Y, Hara J, Kawamura H.
    • Organizer
      3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai, Miyagi, Japan
    • Year and Date
      20090115-20090116
  • [Presentation] エナメル質および象牙質細胞外基質タンパクのシグナル分子としての生物学的機能の解析2009

    • Author(s)
      春山直人
    • Organizer
      第25回 東京医科歯科大学グローバルCOE総合プレゼンテーション
    • Place of Presentation
      東京
    • Year and Date
      2009-05-25
  • [Presentation] 象牙質シアロリン酸化タンパク (DSPP) の象牙質石灰化における役割. 生体バイオマテリアル高機能インターフェイス科学2008

    • Author(s)
      春山直人
    • Organizer
      第3回高機能インターフェイス科学カンファレンス
    • Place of Presentation
      仙台
    • Year and Date
      2008-03-01
  • [Presentation] アメロジェニンのスプライスフォーム (LRAP) を骨特異的に過剰発現させたトランスジェニックマウスにおける骨代謝変化2008

    • Author(s)
      春山直人
    • Organizer
      第29回 東北骨代謝研究会
    • Place of Presentation
      仙台
    • Year and Date
      2008-02-02
  • [Presentation] Characterization of molecular roles of dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) in dentin biomineralization2007

    • Author(s)
      Suzuki S, Haruyama N, Cho A, Honeycutt C, Sreenath TL, Kulkarni AB.
    • Organizer
      9th International Conference on the Chemistry & Biology of Mineralized Tissues
    • Place of Presentation
      Austin, Texas, USA
    • Year and Date
      20071104-20071108
  • [Presentation] Enamel Phenotype and Sexual Dimorphism in Dentin of dspp-null Mouse Molars ASBMR2007

    • Author(s)
      Verdelis K, Wright JT, Haruyama N, Kulkarni AB, Lukashova L, Boskey AL.
    • Organizer
      29th Annual Meeting
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      20070916-20070919
  • [Presentation] Functional analysis of extra cellular matrix proteins as signaling molecules predominantly expressed in tooth enamel and dentin2007

    • Author(s)
      Haruyama N.
    • Organizer
      Tokyo Medical & Dental University Global Center of Excellence Program International Research Center for Molecular Science in Tooth and Bone Diseases 1^<st> Retreat Camp
    • Place of Presentation
      Kanagawa, Japan
    • Year and Date
      20070317-20070318
  • [Book] Overview: engineering transgenic constructs and mice. In : Current Protocols in Cell Biology. 2009 Mar ; Chapter 19 : Unit 19.10(Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwartz J, and Yamada KM editors)2009

    • Author(s)
      Haruyama N, Cho A, Kulkarni AB.
    • Total Pages
      Unit 19.10.1-19.10.9(9)
    • Publisher
      John Wiley & Sons, Inc.
  • [Book] Generation of transgenic mice. In : Current Protocols in Cell Biology. 2009 Mar ; Chapter 19 : Unit 19.11.(Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwartz J, and Yamada KM editors.)2009

    • Author(s)
      Cho A, Haruyama N, Kulkarni AB.
    • Total Pages
      Unit 19.11.1-19.11.22(22)
    • Publisher
      John Wiley & Sons, Inc.

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Published: 2011-06-18   Modified: 2016-04-21  

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