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2021 Fiscal Year Final Research Report

Generation of "infertile fish" to prevent genetic pollution and development of its mass production technology

Research Project

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Project/Area Number 19H03056
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Yoshiura Yasutoshi  国立研究開発法人水産研究・教育機構, 水産技術研究所(廿日市), 主任研究員 (90372052)

Co-Investigator(Kenkyū-buntansha) 吉川 廣幸  国立研究開発法人水産研究・教育機構, 水産大学校, 講師 (40733936)
木下 政人  京都大学, 農学研究科, 准教授 (60263125)
Project Period (FY) 2019-04-01 – 2022-03-31
Keywordsゲノム編集 / 代理親魚法 / 不妊化 / 生殖腺刺激ホルモン受容体 / 全雌生産 / トラフグ / クサフグ / メダカ
Outline of Final Research Achievements

FSH receptor-deficient tiger pufferfish were generated by the CRISPR/Cas9 method with the aim of producing genetically infertile fish. Analysis of female ovaries revealed that all were immature, indicating that FSH receptor deficiency causes infertility in female tiger pufferfish. The DEAD END-deficient grass pufferfish without germ cells was created by genome editing, and then when tiger pufferfish germ cells were transplanted into the DEAD END-deficient grass pufferfish, tiger pufferfish sperm production was observed from these grass pufferfish, indicating that they can be used as surrogate parent fish.

Free Research Field

魚類育種学、魚類生理学

Academic Significance and Societal Importance of the Research Achievements

魚類の不妊化については、その原因遺伝子が特定されてるものは少ない。本研究においてFSH受容体欠損がトラフグでも、雌の不妊化を引き起こし、魚類の雌の不妊化遺伝子として特定できたことは、魚類の生殖生理学において学術的意義は高い。また、不妊化技術は養殖魚の権利保護として産業に貢献する他、逃亡による遺伝子交雑を防ぐため環境保全にもつながる重要な技術であることから、その社会的意義も大きい。

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Published: 2023-01-30  

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