Trial of new therapy development for the peripheral nerve damage by the cell extract derived from a dedifferentiation fat cell

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H03850
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Niigata University
• Principal Investigator: Seo Kenji 新潟大学, 医歯学系, 教授 (40242440)
• Co-Investigator(Kenkyū-buntansha): 田沼 順一 新潟大学, 医歯学系, 教授 (20305139) ; 前田 健康 新潟大学, 医歯学系, 教授 (40183941) ; 岸本 直隆 新潟大学, 医歯学系, 准教授 (50610911) ; 武内 恒成 愛知医科大学, 医学部, 教授 (90206946) ; 紙谷 義孝 岐阜大学, 大学院医学系研究科, 教授 (90381491) ; 山田 友里恵 新潟大学, 医歯学系, 助教 (20804537)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 末梢神経損傷 / シュワン細胞 / 感覚ニューロン / 神経再生 / 脂肪組織由来幹細胞 / 脱分化脂肪細胞 / 細胞抽出物

Outline of Final Research Achievements

Effects of Cell extract from ADSCs and DFATs on peripheral nerve regeneration was investigated in vivo and in vitro. CE-ADSCs increased Schwann cell count and GFAP expression and increased the projection length of the DRG neuron. CE-DFATs increased Schwann cell count and GFAP expression, and they extend the projection of the DRG neuron, but the effect of CE-DFATs tended to have a smaller effect than CE-ADSCs. Three days after CE-ADSCs transplant to the nerve lesion, a macrophage was observed in the proximal site. In addition, an axon extended it towards distal part, and Schwann cell was detected around distal site and the axon. Tough sensation threshold became the level of the same threshold if CE-ADSCs applied to the lesion.
The growth factor included in CE-ADSCs promote axon extension and also activate the non-neuronal cells, resulting in facilitation of nerve regeneration.

Free Research Field

神経科学

Academic Significance and Societal Importance of the Research Achievements

本研究は、末梢神経再生の一つの方法を提案し、そのメカニズムについて科学的に分析した。こうした結果は、将来の組織再生医学に新しい方向性を提案することが出来ると思われる。