Development of genetic manipulation systems and elucidation of gene functions of bacteria that contribute to oral health

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05793
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Kyushu University
• Principal Investigator: MATSUMI Rie 九州大学, 農学研究院, 准教授 (90397597)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 口腔細菌 / 遺伝子操作系 / 超好熱菌 / アーキア / トキシン-アンチトキシンシステム

Outline of Final Research Achievements

To construct a genetic manipulation system for elucidating the gene functions of " bacteria group for oral health ", the toxin-antitoxin (TA) systems from a hyperthermophilic archaea was evaluated. The toxin toxicity was examined by inducing expression of the toxin genes in E. coli. Many of them were highly toxic to E. coli, but most of the antitoxin neutralized the toxicity of the toxins.

Free Research Field

微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究により、超好熱性アーキアのTAシステムが中温性の細菌である大腸菌において機能していることを証明した。今後、本TAシステムを用いて口腔内の健康維持に重要と考えられている細菌群（口腔健康細菌群）の遺伝子操作系の構築し、遺伝子機能を解明することにより口腔予防システムの確立につなげていきたい。