2021 Fiscal Year Final Research Report
Study of genetic polymorphisms to improve fertility by artificial insemination in Japanese Black cattle
Project/Area Number |
19K06370
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 42010:Animal production science-related
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Research Institution | University of Miyazaki |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 黒毛和種 / 受胎率 / 繁殖雌牛 / 人工授精種雄牛 / 遺伝子多型 |
Outline of Final Research Achievements |
The purpose of this study was to search for genetic polymorphisms that affect lower fertility by artificial insemination and to obtain knowledge aimed at improving fertility by artificial insemination through analysis of genetic factors. We analyzed a total of 167 genetic polymorphisms, including polymorphisms reported to be involved in bovine fertility, fertilization system, early development, and based on the results of the comprehensive SNP analysis performed in this study, as well as establishing an analysis protocol using the PCR-RFLP method, which allows genetic analysis at a lower cost. The 28 and 7 genotypes showed significant odds ratios for conception rate of each genotype by the first insemination of each litter in breeding dams and sires used for artificial insemination, with multivariate logistic regression analysis.
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Free Research Field |
家畜遺伝育種学
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Academic Significance and Societal Importance of the Research Achievements |
ウシ人工授精受胎率に関して海外では主に乳用種集団を対象としたGWAS解析により確認されており、同じBos taurus種に属する黒毛和種においても同様の影響を及ぼしている可能性は高いと考えられるが、黒毛和種集団において再現性を確認する必要がある。 本研究では上記に加え、高密度SNPチップによる遺伝子多型情報を収集し、さらに多量の授精記録との遺伝的関連性を解析するにあたり、比較的低コストで実施可能であるPCR-RFLP法により遺伝子多型を解析することで、緊急を要する黒毛和種の受胎性向上のための情報提供を行う、非常に有意義なものであると考えられる。
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