Expression analysis of FGFR in canine mammary tumor

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K06408
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: Yamamoto Masami 日本獣医生命科学大学, 獣医学部, 准教授 (30530026)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: イヌ / 乳腺腫瘍 / FGFR / 乳腺腫瘍細胞株 / real-time RT-PCR / 免疫組織化学染色

Outline of Final Research Achievements

The expression and function of fibroblast growth factor receptor (FGFR) in normal canine tissues and mammary tumors were investigated. It was confirmed that FGFR1-4, which are reported to be expressed in various human cancers and highly expressed in human organs, also showed high RNA expression in dogs. Using canine mammary tumor cell lines and tumor tissues, it was confirmed by real-time RT-PCR analysis that there are cell lines and cases with high expression levels. Additionally, FGFR1-3 were suggested to be associated with the malignancy of tumors, while FGFR4 was suggested to be involved in metastatic potential.

Free Research Field

獣医病理学

Academic Significance and Societal Importance of the Research Achievements

FGFRはヒトにおいてがんの発生母体組織によって、そのサブタイプ1-4の発現量との関連が認められることが報告されている。FGFRはヒト腫瘍に対する新しい分子標的マーカーとしても注目されており、ヒトでの分子標的治療薬として期待されているが、獣医領域ではFGFRの正常ならびに腫瘍での発現量や機能についての報告はほとんどない。本研究によりイヌの正常組織と乳腺腫瘍において各サブタイプのFGFRが発現していることが確認でき、その腫瘍にかかわる機能の一部を明らかにすることができた。