Identification and characterization of the target proteins of anti-inflammatory lipid mediator

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Juntendo University
• Principal Investigator: OKUNO TOSHIAKI 順天堂大学, 大学院医学研究科, 非常勤講師 (60361466)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: SLCO2A1 / CEAS / プロスタグランジン / 慢性腸疾患

Outline of Final Research Achievements

Chronic intestinal diseases associated with the SLCO2A1 gene (CEAS) are caused by loss-of-function mutations in SLCO2A1, which encodes a prostaglandin (PG) transporter. We found a family member who presented to Juntendo Hospital with chronic non-specific ulcers and identified a novel SLCO2A1 mutation by genetic analysis. The levels of urine PG metabolites in patients with ulcers were much increased. We also showed that the novel SLCO2A1 mutation causes loss of PGE2 transporter function and induces CEAS.

Free Research Field

脂質生化学

Academic Significance and Societal Importance of the Research Achievements

本研究では、これまで知られていなかった新規のSLCO2A1の遺伝子変異を同定した。さらに、変異型輸送体に輸送機能がないことを実験的に証明した。本研究で確立した変異型SLCO2A1の機能解析法、及び質量分析計を用いた尿中代謝物の測定は、患者さんの負担の少ない方法で病態の解明や治療法の開発につながることが期待される。