2021 Fiscal Year Final Research Report
Development of the early diagnostic method of intractable ITP using single nucleotide polymorphism analysis of the cytokine gene and EV
| Project/Area Number |
19K07948
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52010:General internal medicine-related
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 量基 関西医科大学, 医学部, 准教授 (70434826)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | ITP / SNP / EV |
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| Outline of Final Research Achievements |
We analized the structure of EV derived from THP-1, the dendritic cell instruction of immunity and cytokine production. The cytokine-related SNP analysis in ITP patients revealed that low frequency of TGF-β1 +869 C/C genotype compared to normal controls. About the treatment reactivity to ITP, a constant tendency was provided in reactivity and cytokine genetic polymorphism of a steroid and the thrombopoietin receptor stimulating medicine (eltrombopag). These results suggested that it was more likely to be tied to early checkup of intractable ITP and the construction of the appropriate treatment strategy by incorporating reactivity of the treatment and EV-related data.
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| Free Research Field |
病態検査学
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| Academic Significance and Societal Importance of the Research Achievements |
ITP患者のTGF-β1 +869 C/C genotype の頻度が健常コントロールに比較して有意に低値であることが判明した。またITPに対する治療反応性に関してもステロイドおよびトロンボポエチン受容体作動薬(エルトロンボパグ)の反応性とサイトカイン遺伝子多型との関連性について新たな知見を得ることができた。活性化したTHP-1からのEVの構造解析、ITPにおける標的細胞の検討と並行して、EVの樹状細胞免疫誘導ならびにサイトカイン産生が明らかとなった。以上より、治療の反応性とEVデータの組み込みによって、難治性ITPの早期診断および適切な治療ストラテジーの構築が可能となることが示唆された。
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