Tooth formation using mouse tooth progenitor cells that can fluorescently label odontoblasts and ameloblasts.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K10041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57010:Oral biological science-related
• Research Institution: Mie University
• Principal Investigator: Yamazaki Hidetoshi 三重大学, 医学系研究科, 教授 (00283987)
• Co-Investigator(Kenkyū-buntansha): 磯野 加奈 三重大学, 医学系研究科, 技術補佐員 (10833858)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: エナメル芽細胞 / 象牙芽細胞 / Amelogenin-X / Dspp / 前駆細胞 / tdTomato / GFP / 胚性幹細胞

Outline of Final Research Achievements

We established Dspp-GFP; Amelx-tdTomato double transgenic mice, that enable us to detect odontoblasts and ameloblasts as GFP-expressing cells or tdTomato-expressing cells fluorescently. Using these mice, GFP+ odontoblasts and tdtomato+ ameloblasts were identified and isolated by immunohistochemistry and flow cytometry. Furthermore, we have established embryonic stem cell lines from Dspp-GFP; Amelx-tdTomato double transgenic mice and attempted to induce GFP+ odontoblasts and tdTomato+ ameloblasts in vitro using different fluorescence markers.
In addition, from these mice, we have identified progenitor cells of odontoblasts or ameloblasts that are negative before culture but become positive for fluorescence after culture by using tooth organ culture.

Free Research Field

発生学、再生医学

Academic Significance and Societal Importance of the Research Achievements

高齢化社会におけるQuality of lifeは、非常に重要であり、特に歯の維持や歯の再生医療は大きな期待をされている。歯はエナメル質を産生するエナメル芽細胞と象牙質を産生する象牙芽細胞からなり、本研究では、初めて、象牙芽細胞特異的遺伝子dentin sialophosphoprotein及びエナメル芽細胞特異的遺伝子Amelogenin の発現を同一個体で別々の蛍光で検出できるマウスや胚性幹細胞株を作成した。これらの系は蛍光を指標にエナメル芽細胞と象牙芽細胞を簡単に検出することができ、今後期待される歯の試験管内再生や治療の有効な道具となる。