Development of 3D cultured skeletal muscle tissue for elucidation of molecular mechanisms of muscle aging

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K11369
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KISHIDA TSUNAO 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205)
• Co-Investigator(Kenkyū-buntansha): 新井 祐志 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (50347449) ; 清水 一憲 名古屋大学, 工学研究科, 准教授 (70402500)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 骨格筋細胞 / ダイレクトコンヴァージョン / サルコペニア / ３次元培養 / 再生医学

Outline of Final Research Achievements

The goal of this study is to establish a fundamental technology that contribute to the elucidation of the molecular mechanisms of sarcopenia. Myoblasts were seeded in a fibrin-based gel, and three-dimensional muscle tissue was formed by several days of differentiation culture using differentiation induction medium. Immunostaining revealed numerous myotubular cells that possess sarcomere structures. An electrical stimulation resulted in contraction of the 3D muscle tissue. The tension generated by the electro-stimulated muscle tissue was measured using a contraction force measurement microdevice, showing that the contraction force tended to increase during the culture period.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

従来の方法よりも優れた骨格筋組織の培養方法を確立することに成功し、得られた骨格筋組織が高い機能を有することを確認できた。（Shimizu K,Kishida T, et al.J Biosci Bioeng. 2020 ）。本研究で作成した骨格筋組織は、筋老化や筋肉の機能においての基礎研究のためのツールとしてのみならず、移植用の骨格筋組織を供給することも期待できるので、外傷や様々な骨格筋組織欠損に対する新しい有効な再生医療法に繋がることが期待される