2020 Fiscal Year Final Research Report
How is the homologous recombination of a gene with repetitive sequence suppressed in bacteria?
| Project/Area Number |
19K15365
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | 反復配列遺伝子 / 相同組換え / バクテリア / Acinetobacter / メチル化修飾 / ナノポアシークエンサー |
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| Outline of Final Research Achievements |
This study aimed to clarify how the Gram-negative bacterium Acinetobacter sp. Tol 5 protects the tandem repeat sequences of the ataA gene from homologous recombination. I constructed a method to measure the frequency of homologous recombination in the ataA gene and compared it between Tol 5 and E. coli. As a result, the frequency of homologous recombination was much lower in Tol 5 than in E. coli. In addition, I successfully isolated a mutant strain with an increased frequency of homologous recombination and identified the mutated gene. We also successfully detected methylation modifications in the tandem repeat sequences of the ataA gene.
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| Free Research Field |
生物工学、分子遺伝学
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| Academic Significance and Societal Importance of the Research Achievements |
相同組換えは、よく似た塩基配列間（相同配列間）で生じるDNAの組換えであり、損傷を受けたDNAの修復機構として生物に普遍的に保存されている。しかし、反復配列に対しても作用することがあり、時に重要な遺伝情報を書き換え、遺伝子本来の機能を失わせてしまう。それにも関わらず、多くの生物のゲノムDNAには相同配列が連続した反復配列が存在する。反復配列の相同組換えを抑制する機構が明らかとなれば、望まない組換えを抑制することや、効率的な遺伝子の書き換えをする技術開発に繋がると期待できる。
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