Optimization of the CRISPR imaging method in mammalian zygote for sex selection

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16026
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42040:Laboratory animal science-related
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Okamura EIichi 滋賀医科大学, 動物生命科学研究センター, 助教 (30755913)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: CRISPRイメージング / 雌雄産み分け

Outline of Final Research Achievements

In animal experiments, only one gender is selectively used from the viewpoint of the reproducibility. Therefore, it is important to develop a technique for sex selection. However, there has been no effective sex selection method that can be applied to mice and rats. In this study, we conceived that visualization of the Y chromosome, which is possessed only by males, in fertilized eggs could be helpful for sex selection. By optimizing the CRIPR imaging technique, which is one of application technologies of genome editing, I succeeded to visualize specific chromosomal region effectively in mouse fertilized eggs.

Free Research Field

発生工学

Academic Significance and Societal Importance of the Research Achievements

本研究ではマウス受精卵において特定の染色体領域を可視化する最適条件を決定することが出来た。この方法を用いてY染色体を可視化すれば、様々な動物種で雌雄産み分けが可能になると考えられる。また、本研究では高効率にTgマウス作製を樹立可能な方法を確立することが出来た。この方法は汎用性があり多くの研究者にとって利便性の高いツールと考えられ、今後の生命科学研究の進展に大きく貢献できると考えられる。