2020 Fiscal Year Final Research Report
Whole genome sequencing analysis of Streptococcus pneumoniae strains to suggest the mechanism of drug resistances and serotype switch based on recombination events
| Project/Area Number |
19K16637
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | 肺炎球菌 / カルバペネム耐性 / マクロライド耐性 / ペニシリン結合タンパク / PBPタイピング |
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| Outline of Final Research Achievements |
Streptococus pneumoniae is one of the major pathogens in community-aquired infections. After the introduction of pneumococcal conjugate vaccines, pneumococci with non-vaccine types and/or antibiotic resistance increased globally. Our study aimed to clary the mechanism of the resisrance and its molecular epidemiology. Our analysis showed that the most of the carbapenem-resistant pneumococci have altered pbp1a (pbp1a-13), suggesting that the gene is cerculating among several clones. With regard to macrolide resistance, we found several tranposon types that had macrolide resistance gene(s). This results indicated that a high rate of macrolide resistance in Japan was caused by not horizontal trasmission of the genes but the antibiotic selection pressure.
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| Free Research Field |
臨床微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は全ゲノム解析を用いて、肺炎球菌ワクチン導入後にどのようなゲノムを持つ肺炎球菌が増加したかを、抗菌薬耐性やワクチン無効の莢膜型の観点から明らかにする研究である。肺炎球菌は遺伝子組み替えを起こしやすい菌であることが知られている。これにより容易にワクチンが効果を示さなくなる。現在肺炎球菌ワクチン開発は莢膜型の疫学情報に基づいて開発が行われている。ゲノムの組み替えのメカニズムや菌の伝播様式が明らかになれば、より効果的なワクチン開発が可能となる。本研究はそのゲノムの組み替えメカニズムや地域をまたいだ伝播様式を明らかにするための基礎的な研究である。
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