Tandem mass spectrometry-based creatinine measurement using dried blood spot for newborn mass screening.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K17371
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52050:Embryonic medicine and pediatrics-related
• Research Institution: Nagoya City University
• Principal Investigator: NAKANO Masaru 名古屋市立大学, 医薬学総合研究院(医学), 助教 (10813579)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 小児 / 腎不全 / 先天性腎尿路奇形 / 新生児マススクリーニング / ろ紙血

Outline of Final Research Achievements

The purpose of this study was to detect Congenital Anomalies of the Kidney and Urinary Tract(CAKUT)
at an early stage. Specifically, we studied the detection of renal urinary tract malformations by detecting decreased renal function in newborns. We have already published a previous study on the ability to measure filter paper blood creatinine levels in newborns using liquid chromatography, the stability of the method, and what would be its cutoff value. This study was planned to measure the number of specimens that could actually withstand screening and to validate the sensitivity and specificity of the method.
Because this study required a large number of specimens, it was conducted with the informal consent of the government agencies. However, at the stage of measurement, they refused to consent to the study. We made a request to other prefectures, but they did not agree to conduct the study, and the study was terminated.

Free Research Field

小児腎臓

Academic Significance and Societal Importance of the Research Achievements

本研究は世界で初めて、新生児期の腎機能スクリーニングを目的としたものであった。本手法は簡便かつ安価で、試薬を追加するだけで可能となるようにセッティングするところまでは到達していた。目標の5000検体での測定には至らなかったものの、200検体での測定精度確認はできている。今後は実際に小児腎不全患者が出た際に、その児の新生児マススクリーニング用ろ紙を用いて測定するなどして、陽性者側から精度を確認する方法を検討している。実際に陽性者発見の可能性を示唆することができれば、再度行政とタイアップしたスクリーニング事業への参画を検討できるものと思われる。