Identification of immune environment cells in the central nervous system which support the development of primary CNS lymphoma

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K17820
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54010:Hematology and medical oncology-related
• Research Institution: University of Tsukuba
• Principal Investigator: Hattori Keiichiro 筑波大学, 医学医療系, 講師 (10832024)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 中枢神経原発悪性リンパ腫

Outline of Final Research Achievements

By RNA sequencing analysis using the nCounter system, we have already performed quantitative RNA analysis of 770 genes related to neuroinflammation in 30 patients with CNS primary malignant lymphoma and compared the results between the poor and good prognosis groups using volcano plots. We found that 48 genes were significantly up-regulated in the poor prognosis group. Furthermore, multivariate analysis revealed that three genes, TUBB4A, S100B, and SLC6A1, had a significant effect on prognosis.

Free Research Field

造血器疾患

Academic Significance and Societal Importance of the Research Achievements

前述の3つの遺伝子は、オリゴデンドロサイトなどの中枢神経の免疫環境に関連していたことから、PCNSLの免疫環境細胞が臨床予後に関係している可能性があることが示唆される。シングルセルシークエンス解析を通じて更に詳細な解析を行うことで、予後予測のためのバイオマーカーの同定につながり、将来的に創薬につながっていくことが期待される。