A novel prophylaxis for gut GVHD through mirobiota analysis

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K17828
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54010:Hematology and medical oncology-related
• Research Institution: Kyoto University
• Principal Investigator: Arai Yasuyuki 京都大学, 医学研究科, 助教 (10826564)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 腸内細菌叢

Outline of Final Research Achievements

This study can be divided into (1) collection and identification of intestinal microbiota after bone marrow transplantation for hematopoietic tumors, (2) classification of intestinal microbiota by machine learning using AI, and integration and analysis with post-transplant clinical database, (3) similar investigation in mouse experimental models and prevention experiments using live bacteria preparations, and preparation for human clinical trials based on these studies. The first is the collection of intestinal microbiota.
The collection of intestinal microbiota in (1) will be conducted weekly for 2 months from the start of the pre-transplantation treatment.
The patient's stool samples will be collected weekly for 2 months from the start of the pre-transplantation treatment. The nucleotide sequence of the 16S ribosomal RNA-coding gene, which is a gene specific to the bacteria, will be determined using a next-generation sequencer.

Free Research Field

造血幹細胞移植

Academic Significance and Societal Importance of the Research Achievements

検体採取は順調に進行し、これまでに合計21患者を対象とし、100サンプル以上を検討した。予定したプロトコールによって、患者からの同意取得、検体の採取、院内検査室への搬送、解析研究室への輸送、検体の保存、次世代シーケンスを用いた測定、コンピュータを用いた解析には大きな問題は発生せず、予定通り進捗している。結果は、属レベルまでの細菌分類が可能であった。