Transcriptional regulation of the fibrillar collagen genes (type V/XI/XXIV/XXVII) in osteoblasts and chondrocytes

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K18469
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Oita University
• Principal Investigator: Hida Mariko 大分大学, 医学部, 客員研究員 (10737224)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: コラーゲン / 骨 / 軟骨 / 転写

Outline of Final Research Achievements

The fibrillar collagen genes are major components of the extracellular matrix. They has an important role in regulating the bone formation. This study analyzed the transcriptional regulation of these fibrillar collagen genes(type V/XI/XXIV/XXVII) in osteoblasts and chondrocytes.
Type XI and XXVII collagen genes indicated the cartilage-specific enhancer regions in chondrocytes. However, there are different transcriptional regulation systems between type XI and XXVII collagen genes. The promoter region of type XI collagen gene is activated by transcriptional factor Sp1 but, the promoter region of type XXVII collagen gene is not activated by Sp1 though type XXVII collagen gene has same sequences like GC-rich.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

線維性コラーゲン分子は、発生や分化・再生過程において、機能維持の制御といった重要な役割を担っているため、適切な細胞環境が維持されていない場合、骨・軟骨形成異常等の疾患を引き起こす要因となる。そこで、骨・軟骨組織における転写調節機構の解析を行うとともに関与する転写因子の同定を行い、その作用機序を解明することによって、疾患の早期の診断や治療への応用に役立つ新たな知見を得ることができるほか、再生医療への応用へもつながるのではないかと考える。