2022 Fiscal Year Final Research Report
The effect of Nrf2 on the intercellular adhesion in gingival junctional epithelial cells
| Project/Area Number |
19K19034
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57030:Conservative dentistry-related
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 酸化ストレス / 歯肉上皮 / 抗酸化 / 細胞接着 |
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| Outline of Final Research Achievements |
In this study, using a human gingival epithelial cell line (Ca9-22 cell) and a mouse junctional epithelial cell line (JE-1 cell) established in our laboratory, we studied that the oxidative stress regulator Nrf2 affects intercellular adhesion of gingival epithelial cells. We also investigated the effect of sulforaphane, an Nrf2 inducer.
As a result, it was suggested that sulforaphane may promote Nrf2 nuclear translocation and exhibit antioxidant effects after oxidative stimulation by H2O2 in Ca9-22 cells. It was also suggested that sulforaphane may maintain the epithelial barrier function by affecting the expression of a part of the intercellular adhesive molecule of gingival epithelial cells.
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| Free Research Field |
歯周病学
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| Academic Significance and Societal Importance of the Research Achievements |
歯周病の罹患率は高齢化に伴って年々増加し、今や国民病とも言われている。歯周病はバイオフィルムによる慢性炎症性疾患であり、症状のないまま緩慢に進行するため歯科受診時にはすでに中等度以上進行している場合も多い。歯周病原細菌の生体内への侵入プロセスには、細菌側の病原因子だけでなく、生体側、特に歯肉上皮の防御機構の破綻が生じる。
本研究成果により、感染防御の最前線である歯肉上皮細胞の物理的バリア機能を司る細胞間接着機構についての解明が進むことは、その維持や修復を目的とした新しい予防・治療法の発見につながると期待される。
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