Comprehensive epigenetic analysis of periodontal pathogenic bacteria in the kidney - To prevent underlying diseases-

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21371
• Project/Area Number (Other): 18H06282 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0907:Oral science and related fields
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: HARADA Fumiya 北海道医療大学, 歯学部, 助教 (30824205)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: 歯周病原菌由来LPS / 慢性腎不全

Outline of Final Research Achievements

In this study, we analyzed gene expression changes in kidney tissues induced by periodontal pathogen derived LPS (PG-LPS) to elucidate the interrelation between periodontal disease and chronic kidney disease (CKD). Repeated intraperitoneal injection of PG-LPS were performed to normal and senescence-accelerated (SAMP) mice for 3 months, and the suspicion of nephritis was significantly higher in LPS-treated normal and SAMP mice than in the controls. LPS receptor expression analysis indicated that LPS promotes inflammation of the kidney by acting not only on the glomerulus but also on the tubules. Also, expression level of inflammation relating genes were up-regulated significantly.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

これまでPG-LPSによる腎臓の遺伝子発現変化を観察した報告は僅かであり、本研究成果はCDKの発症に歯周病原菌が関与していることを示唆するデータとなる。現在CKD罹患者数は増加の一途をたどっている。本研究成果を基に口腔衛生指導をより強化することでCKD予防に寄与することが示唆された。これまでのCKDに対する治療法に新たな手段を加える研究として、意義ある内容である。