Pathologic analysis of retroviral cardiomyocyte abnormality and the usefulness as an animal disease model

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22356
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 42:Veterinary medical science, animal science, and related fields
• Research Institution: Iwate University
• Principal Investigator: Ochiai Kenji 岩手大学, 農学部, 教授 (80214162)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: 鳥白血病ウイルス / 先天性血管腫 / iPS細胞 / 無限分裂細胞 / RNA-seq

Outline of Final Research Achievements

To elucidate the pathogenesis of cardiomyocyte abnormality caused by avian leukosis viruses, iPS cells were produced from chick embryo. But, these cells were unuseful for further RNA-seq analysis because the cells were infected with multiple ALV strains. Meanwhile, immortalized cell lines derived from multiple congenital hemangioma in a Japanese Black calf was established. RNA-seq analysis demonstrated TNF signaling pathway anomalies and over expression of TNFRSF6B gene in the cell lines.

Free Research Field

獣医病理学

Academic Significance and Societal Importance of the Research Achievements

iPS細胞および無限分裂細胞は異常な細胞を生きたまま保存できることから，原因不明の疾病研究や発がん機序解明への応用が期待されているが，ヒトを含め成功例は少ない。そこで，今回レトロウイルス性腫瘍からiPS細胞を，非ウイルス性腫瘍から無限分裂細胞を作製し解析を試みた。ウイルス性腫瘍の解析には改良が必要であるが，少なくとも無限分裂細胞化技術は動物疾病，特に稀な動物腫瘍の細胞レベルの解析を容易にすることから，今後の応用が期待される。