A novel intraoperative infection detecting liquid for medication-related osteonecrosis of the jaw

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K24093
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Kobe University
• Principal Investigator: Arimoto Satomi 神戸大学, 医学部附属病院, 医員 (20841042)
• Project Period (FY): 2019-08-30 – 2023-03-31
• Keywords: 顎骨壊死 / う蝕検知液

Outline of Final Research Achievements

This study aims to study the effect of various dyes for detecting the infection bone area and then develop a novel intraoperative infection detecting liquid for medication-related osteonecrosis of the jaw (MRONJ). First, we performed preliminary experiment using five dyes by a rat MRONJ model. Rats were randomly assigned into six groups: Undyed, Acid red, Brilliant blue, Phloxine group, Methylene blue and Rose bengal group. Based on visual image and histological results, we selected Brilliant blue and Phloxine. Second, female SD rats were divided between Control groups, Undyed, Brilliant blue, and Phloxine. After establishing the MRONJ condition, we applied each dye. Stained area completely removed and the bone defect was closed suture. We were examined that soft tissue evaluation, histological analysis and micro-computed tomography (CT) analysis.These results indicated Phloxine dyes could distinguish necrotic bone area from normal bone area during surgery.

Free Research Field

外科系歯学

Academic Significance and Societal Importance of the Research Achievements

本研究課題の核心をなす学術的「問い」は，①染色液がMRONJの感染骨可視化を可能とするか，②正確な術中切除範囲を決定し，病理組織学的に感染骨を除去可能かの2点である．