2010 Fiscal Year Final Research Report
The analysis of the role of spatio-temporal dynamics of intracellular proteins in cell responses
| Project/Area Number |
20200057
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
Morphology/Structure
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| Research Institution | Tohoku University |
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Principal Investigator |
OHASHI Kazumasa 東北大学, 大学院・生命科学研究科, 准教授 (10312539)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 細胞構造・機能 / 細胞骨格 / 情報伝達 / イメージング技術 / アクチン / コフィリン |
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| Research Abstract |
Actin cytoskeleton dynamics are regulated by a variety of actin-binding proteins, which cooperatively act in the assembly/disassembly and reorganization of actin filaments in cells. In this study, we have assessed the concentration of actin monomer or cofilin in the cytoplasm of living cells by the fluorescence decay after photobleaching (FDAP) assay. We found that cofilin is involved in the generation of more than half of the actin monomers in the cytoplasm. We also found that cofilin exhibits fast turnover between cytoplasm and actin filaments. These results suggest that the degree of dynamics of actin or cofilin determines the capacity of cell motility.
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Research Products
(29 results)
