2010 Fiscal Year Final Research Report
The role of miRNA in the pain mechanisms
| Project/Area Number |
20390417
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
HAGIHIRA Satoshi Osaka University, 医学部附属病院, 講師 (90243229)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAE Aya 大阪大学, 医学系研究科, 特任准教授(常勤) (60379170)
NAKAI Kunihiro 大阪大学, 医学系研究科, 寄附講座准教授 (80362705)
SHIBATA Masahiko 大阪大学, 医学系研究科, 寄附講座教授 (50216016)
TAKASHINA Masaki 大阪大学, 医学部附属病院, 講師 (30221352)
MASHIMO Takashi 大阪大学, 医学系研究科, 教授 (10110785)
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| Project Period (FY) |
2008 – 2010
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| Keywords | マイクロRNA / GABA受容体 / セロトニン受容体 |
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| Research Abstract |
In the current study, we investigated the function of multiple miRNAs, focusing on those that are considered potential targets for GABAA alpha 5 receptor (Gabra5) regulation.
The in silico approach identified six miRNAs that were candidates for Gabra5 regulation (miR 598-3p, miR541, miR378, miR219-2-3p, miR223, and miR346). Luciferase assays confirmed that the six miRNAs targeted specific sequences within Gabra5. We next examined whether these miRNAs reduced Gabra5 expression at the protein level. We found that only miR378 and miR541 negatively regulated Gabra5 protein in hippocampal neurons.
Gabra5 plays an important role in cognition and memory. By increasing the expression level of Gabra5, we might be able to ameliorate the symptoms of patients with Alzheimer's disease, who suffer from cognitive problems and memory dysfunction. Inhibitors of miR378 and miR541 are potential targets for this putative new therapy.
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[Journal Article] Recovery of motoneuron and locomotor function after chonicspinal cord injury depends on constitutive activity in 5-HT2C receptors.2010
Author(s)
Murray K, Nakae A, Stephens MJ, Rank M, D'Amico J, Harvey P, Li X, Harris L, Ballou EW, Anelli R, Heckman CJ, Mashimo T, Vavrek R, Sanelli L, Gorassini MA, Bennett DJ, Fouad K.
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Journal Title
Nature Medicine 16(8)
Pages: 694-700
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[Journal Article] Role of constitutively active 5HT2C receptors following spinal cord injury in rats.2008
Author(s)
K.C. Murray, A. Nakae, T. Ballou, R. Vavrek, K. Fouad, M.M. Rank, M. Stephens, R. Anelli, PJ. Harvey, C. Heckman, D.J. Bennett
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Journal Title
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[Presentation] The snoRNA RBII-52 regulates alternative splicing of serotonin 2C receptor in the rat oro-facial neuropathic paimodel.2009
Author(s)
Aya Nakae, Kunihiro Nakai, Tatsuya Tanaka, Mari Yoshida, Akiko Mikam, Masaki Takashina, Satoshi Hagihira, Masahiko Shibata, Koichi Ueda, Takashi ashimo
Organizer
Neuroscience 2009 poster
Place of Presentation
Chicago, Ilinoi
Year and Date
20091017-20091021
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[Presentation] Role of constitutively active 5HT2C receptors following spinal cord injury in rats.2008
Author(s)
K.C. Murray, A.Nakae, T. Ballou, R. Vavrek, K. Fouad, M.M. Rank, M. Stephens, R. Anelli, P.J. Harvey, C. Heckman, D.J. Bennett
Organizer
Neuroscience 2008
Place of Presentation
Washington, DC
Year and Date
20081115-20081119
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