Effects of neonatal exposure to thyroid hormone disrupting chemicals in rats

2010 Fiscal Year Final Research Report

• Project/Area Number: 20510065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Hiroshima University
• Principal Investigator: FUJIMOTO Nariaki 広島大学, 原爆放射線医科学研究所, 准教授 (40243612)
• Co-Investigator(Kenkyū-buntansha): KITAMURA Shigeyuki 日本薬科大学, 薬学部, 教授 (40136057) ; SUGIHARA Kazumi 広島大学, 医歯薬総合研究科, 准教授 (20271067)
• Project Period (FY): 2008 – 2010
• Keywords: 甲状腺ホルモン / 内分泌かく乱物質 / 子供の環境リスク

Research Abstract

The expression of rat liver genes was determined by a cDNA microarray method to identify the thyroid hormone responsive genes. The identified genes include RLT3-2, RLT3-13, RLT3B-24 and RLT3B-49. In rats, neonatal exposure to thyroid hormones irreversibly altered the expression profile of these genes in adulthood, which suggested these genes could be useful markers for the neonatal effects of thyroid hormone disrupting chemicals. In addition, we established an improved thyroid hormone reporter gene assay to determine environmental chemicals exhibiting weak thyroid hormone activity.

Research Products

• [Journal Article] Animproved thyroid hormone reporter assay to determine the thyroid hormone-likeactivity of amiodaronebithionol, closantel and rafoxanide (2012)
  • Author(s): Matsubara K., Sanoh S., Ohta S., Kitamura S., Sugihara K., Fujimoto N.
  • Journal Title: Toxicol. Lett Volume: 208 Pages: 30-35
  • Peer Reviewed
• [Journal Article] Androgen dependent transcription of amouse prostatic protein gene, PSP94 : involvement of estrogen receptors (2011)
  • Author(s): Fujimoto N., Kitamura S., Kanno J.
  • Journal Title: JSteroid Biochem. Mol. Biol Volume: 127 Pages: 301-306
  • Peer Reviewed
• [Journal Article] Identification of ratprostatic secreted proteins using masss pectrometric analysis andandrogen-dependent mRNA expression (2009)
  • Author(s): Fujimoto, N., Suzuki, T., Ohta, S., Kitamura, S.
  • Journal Title: J. Androl. Volume: 30 Pages: 669-678
  • Peer Reviewed
• [Journal Article] Affinity for ThyroidHormone and Estrogen Receptors of Hydroxylated Polybrominated Diphenyl Ethers. (2008)
  • Author(s): Kitamura, S., Shinohara, S., Iwase, S., Sugihara, K., Uramaru, N., Shigematsu, N., Fujimoto, N., Ohta, S.
  • Journal Title: J. Health Science Volume: 54 Pages: 607-614
  • Peer Reviewed
• [Journal Article] Metabolicactivation of proestrogens in theenvironment by cytochrome P450 system (2008)
  • Author(s): Kitamura, S., Sugihara K., Sanoh, S., Fujimoto, N., Ohta, S.
  • Journal Title: J. Health Science Volume: 54 Pages: 343-355
  • Peer Reviewed
• [Presentation] 環境化学物質の甲状腺ホルモン攪乱作用in vitro評価 (2011)
  • Author(s): 松原加奈, 佐能正剛, 藤本成明, 浦丸直人, 杉原数美, 北村繁幸, 太田茂
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 20110300
• [Presentation] 低用量TCDD新生児期暴露のマウス前立腺分泌機能への影響 (2010)
  • Author(s): 藤本成明, 高木篤也, 菅野純
  • Organizer: 環境ホルモン学会第13回研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-17
• [Presentation] 環境化学物質甲状腺ホルモン作用の高感度レポータージーンアッセイ (2010)
  • Author(s): 松原加奈, 藤本成明, 佐能正剛, 杉原数美, 北村繁幸, 太田茂
  • Organizer: 環境ホルモン学会第13回研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-16
• [Presentation] マウスPSP94のアンドロゲン応答性転写機構.エストロゲン受容体の関与 (2010)
  • Author(s): 藤本成明, 北村繁幸
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Requirement of estrogen receptorαforandrogen dependent transcription of amouse prostatic protein gene, PSP94 (2010)
  • Author(s): Fujimoto N., Kitamura S., Ohta, S.
  • Organizer: 14thInternational Congress on HormonalSteroids and Hormones & Cancer, Edinburgh
  • Place of Presentation: Edinburgh,U. K.
  • Year and Date: 2010-09-23
• [Presentation] 環境化学物質による甲状腺ホルモン応答の高感度評価 (2010)
  • Author(s): 松原加奈, 佐能正剛, 岩瀬恵理, 藤本成明, 浦丸直人, 杉原数美, 加藤善久, 北村繁幸, 太田茂
  • Organizer: フォーラム2010衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] 環境化学物質のproteindisulfide isomerase(PDI)への結合による内分泌系に対する影響 (2010)
  • Author(s): 佐能正剛, 松原加奈, 岩瀬恵理, 今岡進, 岡田和嗣, 浦丸直人, 藤本成明, 杉原数美, 北村繁幸, 太田茂
  • Organizer: フォーラム2010衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-09
• [Presentation] 環境中における甲状腺ホルモン撹乱物質のスクリーニング (2010)
  • Author(s): 佐能正剛, 岩瀬恵理, 松原加奈, 杉原数美, 浦丸直人, 藤本成明, 北村繁幸, 太田茂
  • Organizer: 130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-30
• [Presentation] 化学物質による肝肥大の毒性指標としての性ホルモン受容体および応答性遺伝子発現の検討 (2010)
  • Author(s): 藤本成明, 吉田緑, 西川秋佳, 小澤正吾, 蒲生俊恵, 根本清光, 出川雅邦
  • Organizer: 第26回日本毒性病理学会学術集会
  • Place of Presentation: 金沢
  • Year and Date: 2010-02-03
• [Presentation] MS解析による前立腺タンパク質の同定とそのアンドロゲン応答性 (2009)
  • Author(s): 藤本成明, 鈴木智晴, 太田茂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 環境化学物質の甲状腺ホルモン受容体結合活性とその代謝変動 (2009)
  • Author(s): 佐能正剛, 岩瀬恵理, 杉原数美, 加藤善久, 浦丸直人, 藤本成明, 北村繁幸, 太田茂
  • Organizer: 環境ホルモン学会第12回研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2009-12-07
• [Presentation] Prostatic secreted proteins in mice andrats : Identification using massspectrometric analysis and the hormonede pendent expression. (2009)
  • Author(s): Fujimoto N., Suzuki, T., Ohta, S.
  • Organizer: European Congressof Endocrinology
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2009-04-28
• [Presentation] 臭素化難燃剤PBDEの甲状腺ホルモン受容体結合活性の代謝による変動 (2009)
  • Author(s): 岩瀬恵理, 小島弘幸, 杉原数美, 浦丸直人, 藤本成明, 黒木広明, 北村繁幸, 太田茂
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-27
• [Presentation] 出川雅邦化学物質による肝肥大の毒性指標としての性ホルモン受容体発現の検討 (2009)
  • Author(s): 藤本成明, 吉田緑, 西川秋佳, 根本清光, 出川雅邦
  • Organizer: 第25回日本毒性病理学会学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2009-01-28
• [Presentation] マウス前立腺におけるアンドロゲン応答遺伝子同定とホルモン存性前立腺増殖への関与 (2008)
  • Author(s): 藤本成明, 菅野純, 五十嵐勝秀
  • Organizer: 日本癌学会第67回学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Book] T. Satoh, R. C. Gupta(ed.) Anticholinesterase Pesticides, John Wiley & Sons, Ltd, Hoboken, NJ (2010)
  • Author(s): Kitamura, S., Sugihara, K., Fujimoto, N., Yamazaki, T.
  • Total Pages: 203-223
  • Publisher: Organophosphates asendocrine disruptors In
• [Book] Endocrine Disruption in Toxic Responses, B. Ballantyne, T. Marrs, T. Syversen(ed.) General and AppliedToxicology III, John Wiley & Sons, Ltd, Chichester (2009)
  • Author(s): Kitamura, S., Sugihara, K., Nakamura, K., Kotake, K., Kashiwagi, A., Fujimoto, N.
  • Total Pages: 539-582