Study on the network among metal-related proteins for mechanisms and functions of vanadium ions.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570070
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Hiroshima University
• Principal Investigator: UEKI Tatsuya Hiroshima University, 大学院・理学研究科, 准教授 (10274705)
• Project Period (FY): 2008 – 2010
• Keywords: ホヤ / バナジウム / 金属結合タンパク質 / 金属輸送体 / タンパク質間相互作用

Research Abstract

In order to reveal protein-protein interaction network among Vanabins and metal-related proteins in vanadocytes and blood plasma in a vanadium-rich ascidian Ascidia sydneiensis samea, we performed the screening for proteins that interact with known vanadium-binding proteins, and analyzed protein-protein interaction and metal-binding and -transfer activities.

Research Products

• [Journal Article] A novel vanadium transporter of the Nramp family expressed at the vacuole of vanadium-accumulating cells of the ascidian Ascidia sydneiensis samea. (2011)
  • Author(s): Ueki, T., Furuno, N., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1810 Pages: 457-464
  • Peer Reviewed
• [Journal Article] Molecular mechanism of the transport and reduction pathway of vanadium in ascidians. Coord. (2011)
  • Author(s): Ueki, T., Michibata, H.
  • Journal Title: Chem.Rev. in press.
  • Peer Reviewed
• [Journal Article] Advances in research on the accumulation, redox behavior, and function of vanadium in ascidians. (2010)
  • Author(s): Michibata, H., Ueki, T.
  • Journal Title: Biomol. Concep. 1 Pages: 97-107
  • Peer Reviewed
• [Journal Article] Characterization of vanadium-binding sites of the vanadium-binding protein Vanabin2 by site-directed mutagenesis. (2009)
  • Author(s): Ueki, T., Kawakami, N., Toshishige, M., Matsuo, K., Gekko, K., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1790 Pages: 1327-1333
  • Peer Reviewed
• [Journal Article] Identification and biochemical analysis of a homolog of a sulfate transporter from a vanadium-rich ascidian Ascidia sydneiensis samea. (2009)
  • Author(s): Ueki, T., Furuno, N., Xu, Q., Nitta, Y., Kanamori, K., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1790 Pages: 1295-1300
  • Peer Reviewed
• [Journal Article] A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer. (2009)
  • Author(s): Kawakami, N., Ueki, T., Amata, Y., Kanamori, K., Matsuo, K., Gekko, K., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1794 Pages: 674-679
  • Peer Reviewed
• [Journal Article] Sequence variation of Vanabin2-like vanadium-binding proteins in blood cells of the vanadium-accumulating ascidian Ascidia sydneiensis samea, Biochim. (2008)
  • Author(s): Ueki, T., Satake, M., Kamino, K., Michibata, H.
  • Journal Title: Biophys. Acta 1780 Pages: 1010-1015
  • Peer Reviewed
• [Presentation] Molecular mechanism of the transport and reduction pathway of vanadium in ascidians. (2010)
  • Author(s): Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム,招待講演
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] Expressed sequence tag (EST) analysis of the intestine from the most vanadium-rich ascidian Ascidia gemmata, and their application to heavy metal biosorption system. (2010)
  • Author(s): Samino, S., Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] Measurement and comparison of V(V)-reductase activity of Vanabin family of a vanadium-rich ascidian Ascidia sydneiensis samea. (2010)
  • Author(s): Kimizu, T., Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] Identification of genes regulated by excess vanadium ions in ascidians, focusing on redox and accumulation of metals. (2010)
  • Author(s): Kume, S., Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム,ポスター賞受賞
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer. (2009)
  • Author(s): Michibata, H., Ueki, T.
  • Organizer: 第14回国際生物無機化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-25
• [Presentation] A novel vanadium transporter of the Nramp/DCT family from a vanadium-rich ascidian Ascidia sydneiensis samea. (2009)
  • Author(s): Ueki, T., Michibata, H.
  • Organizer: 第14回国際生物無機化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-25
• [Presentation] Protein-protein interactions among vanadium-binding proteins and related proteins in a vanadium-rich ascidian. (2008)
  • Author(s): Ueki, T., Michibata, H.
  • Organizer: 第6回国際バナジウムシンポジウム
  • Place of Presentation: リスボン(ポルトガル)
  • Year and Date: 2008-07-17
• [Book] Vanadium compounds/vanadate oligomers in biological systems : Chemistry, biochemistry and biological effects Research Signpost Inc (2008)
  • Author(s): Ueki, T., Michibata, H.
  • Total Pages: 356
• [Remarks] ホームページ等
  • URL: http://home.hiroshima-u.ac.jp/bio/APN/index.html