Studies on the Bioactivity and Intensive Use of the Constituents of Azadirachta indica (Neem)

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical pharmacy
• Research Institution: Nihon University
• Principal Investigator: AKIHISA Toshihiro Nihon University, 理工学部, 教授 (30060213)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Takashi 日本大学, 薬学部, 教授 (40318457) ; YASUKAWA Ken 日本大学, 薬学部, 教授 (00130625)
• Project Period (FY): 2008 – 2010
• Keywords: ニーム / リモノイド / メラニン産生抑制 / 抗腫瘍活性 / 発がん予防効果

Research Abstract

Thirty-one limonoids and one diterpenoid, and seventeen limonoids were isolated from the methanol and hexane extracts, respectively, of neem seeds. Among these compounds, seven were new compounds, and their structures were determined spectroscopically. These compounds were evaluated for their melanogenesis-inhibitory, anti-inflammatory, Epstein-Barr virus early antigen (EBV-EA) activation inhibitory, and cytotoxic activities. Some of these compounds have been revealed to possess potent melanogenesis-inhibitory, anti-inflammatory, EBV-EA activation inhibitory, and cytotoxic activities with apoptosis induction.

Research Products

• [Journal Article] Cytotoxic and apoptosis-inducing activities of limonoids from the seeds of Azadirachta indica (neem) (2011)
  • Author(s): Takashi Kikuchi, Koichi Ishii, Taisuke Noto, Akitomo Takahashi, Keiichi Tabata, Takashi Suzuki, Toshihiro Akihisa
  • Journal Title: Journal of Natural Products 74巻 Pages: 866-870
  • Peer Reviewed
• [Journal Article] The melanogenesis-inhibitory, antiinflammatory, and chemopreventive effects of limonoids from n-hexane extract of Azadirachta indica A.Juss. (neem) seeds (2011)
  • Author(s): Toshihiro Akihisa, Akitomo Takahashi, Takashi Kikuchi, Mio Takagi, Kensuke Watanabe, Makoto Fukatsu, Yukiko Fujita, Norihiro Banno, Harukuni Tokuda, Ken Yasukawa
  • Journal Title: Journal of Oleo Science 60巻 Pages: 53-59
  • Peer Reviewed
• [Journal Article] Melanogenesis inhibitory, anti-inflammatory, and chemopreventive effects of limonoids from the seeds of Azadirachta indica A.Juss. (neem) (2009)
  • Author(s): Toshihiro Akihisa, Taisuke Noto, Akitomo Tkahashi, Yukiko Fujita, Norihiro Banno, Harukuni Tokuda, Kazuo Koike, Takashi Suzuki, Ken Yasukawa, Yumimko Kimura
  • Journal Title: Journal of Oleo Science 58巻 Pages: 581-594
  • Peer Reviewed
• [Presentation] Cytotoxic and apoptosis -inducing activities of limonoids from the seed extract of Azadirachta indica A.Juss. (neem) (2011)
  • Author(s): Takashi Kikuchi, Koichi Ishii, Taisuke Noto, Akitomo Takahashi, Keiichi Tabata, Takashi Suzuki, Toshihiro Akihisa
  • Organizer: The 3rd International Conference on Natural Products for Health and Beauty (3rd NATPRO)
  • Place of Presentation: The Emerald Hotel Bangkok
  • Year and Date: 2011-03-18
• [Presentation] ニーム種子ヘキサン抽出物由来リモノイド成分の生物活性 (2010)
  • Author(s): 渡邊賢介,高橋章友,菊地崇,高木美緒,秋久俊博,深津誠,藤田幸子,伴野規博,徳田春邦,安川憲
  • Organizer: 日本生薬学会第57回年会
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2010-09-26
• [Presentation] ニーム葉部リモノイド成分の細胞傷害活性 (2009)
  • Author(s): 高木美緒,高橋章友,石井孝一,菊地崇,伴野規博,鈴木孝, 秋久俊博
  • Organizer: 第53回日本大学理工学部学術講演会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2009-11-28
• [Presentation] ニーム種子由来リモノイド類化合物の細胞傷害活性及びメラニン産生抑制活性 (2009)
  • Author(s): 高橋章友,菊地崇,渡邊賢介,伴野規博,深津誠,田畑恵市,鈴木孝,秋久俊博
  • Organizer: 第53回日本大学理工学部学術講演会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2009-11-28
• [Presentation] ニーム種子由来リモノイド化合物のアポトーシス誘導活性 (2009)
  • Author(s): 石井孝一,能登泰輔,二瓶雅俊,高橋章友,高木美緒,秋久俊博,田畑恵市,鈴木孝
  • Organizer: 日本生薬学会第56回年会
  • Place of Presentation: 京都薬科大学
  • Year and Date: 2009-10-04
• [Presentation] ニーム葉部リモノイド成分の細胞傷害および抗炎症活性,ならびにEBV 活性化抑制効果 (2009)
  • Author(s): 高木美緒,秋久俊博,高橋章友,石井孝一,菊地崇,伴野規博,安川憲,徳田春邦
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-28
• [Presentation] ニーム種子抽出物不ケン化脂質の新規ノルトリテルペノイド成分 (2008)
  • Author(s): 高橋章友,秋久俊博,能登泰輔,菊地崇,伴野規博,藤田幸子,鈴木孝,安川憲,木村由美子
  • Organizer: 第52回日本大学理工学部学術講演会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2008-11-29
• [Presentation] ニーム葉部リモノイド成分の単離および細胞傷害活性 (2008)
  • Author(s): 高木美緒,秋久俊博,高橋章友,石井孝一,菊地崇,伴野規博,孫奕,安川憲,木村由美子
  • Organizer: 第52回日本大学理工学部学術講演会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2008-11-29
• [Presentation] ニーム葉部のリモノイド成分 (2008)
  • Author(s): 高木美緒,高橋章友,能登泰輔,秋久俊博,孫奕,安川憲,木村由美子
  • Organizer: 日本油化学会第47回年会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2008-09-17
• [Presentation] ニーム種子由来成分のメラニン産生抑制活性 (2008)
  • Author(s): 高橋章友,秋久俊博,能登泰輔,伴野規博,藤田幸子,鈴木孝,木村由美子
  • Organizer: 日本油化学会第47回年会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2008-09-17