Identification of iron transporter for mitochondrial iron accumulation in myelodysplastic syndrome.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Asahikawa Medical College
• Principal Investigator: TORIMOTO Yoshihiro Asahikawa Medical College, 医学部, 准教授 (00281882)
• Co-Investigator(Kenkyū-buntansha): SATO Kazuya 旭川医科大学, 医学部, 講師 (50360988) ; IKUTA Katsuya 旭川医科大学, 医学部, 講師 (00396376)
• Project Period (FY): 2008 – 2010
• Keywords: 貧血 / ミトコンドリア / 鉄 / トランスポーター / ABCB7遺伝子 / 骨髄異形成症候群

Research Abstract

In this study, we identified the iron transporter gene for mitochondrial iron accumulation in myelodysplastic syndrome (MDS) using bone marrow cells from refractory anemia with ringed sideroblasts (RARS). The decreased expression of ABCB7 gene, which is located on Xq13 and is involved in mitochondrial iron-transport to the cytosol, was observed in the bone marrow cells in the RARS patient with abnormal X-chromosome. Moreover, the levels of ABCB7 mRNA in RARS patients with normal chromosome were also decreased. These findings suggested that the abnormal ABCB7 gene expression contributes to the pathogenesis of mitochondrial iron accumulation in adult MDS patients.

Research Products

• [Journal Article] Loss of ABCB7 gene : pathogenesis of mitochondrial iron accumulation in erythroblasts in refractory anemia with ringed sideroblast with isodicentric (X)(q13). (2011)
  • Author(s): Sato K, Torimoto Y, Hosoki T, Ikuta K, Takahashi H, Yamamoto M, Ito S, Okamura N, Ichiki K, Tanaka H, Shindo M, Hirai K, Mizukami Y, Otake T, Fujiya M, Sasaki K, Kohgo Y
  • Journal Title: Int J Hematol. 93 Pages: 311-318
  • Peer Reviewed
• [Journal Article] Detection of soluble HFE associated with soluble transferrin receptor in human serum. (2011)
  • Author(s): Ohnishi K, Torimoto Y, Ikuta K, Tanaka H, Hosoki T, Tanaka S, Hamano A, Sato K, Fujiya M, Sasaki K, Kohgo Y
  • Journal Title: Int J Mol Med 27 Pages: 435-439
  • Peer Reviewed
• [Journal Article] Heterogeneous expressions of hepcidin isoforms in hepatoma-derived cells detected using simultaneous LC-MS/MS. (2009)
  • Author(s): Hosoki T, Ikuta K, Shimonaka Y, Sasaki Y, Yasuno H, Sato K, Ohtake T, Sasaki K, Torimoto Y, Sato K, Kohgo Y
  • Journal Title: Proteomics Clin Appl. 3 Pages: 1256-1264
  • Peer Reviewed
• [Journal Article] Body iron metabolism and pathophysiology of iron overload. (2008)
  • Author(s): Kohgo Y, Ikuta K, Ohtake T, Torimoto Y, Kato J
  • Journal Title: Int J Hematol. 88 Pages: 7-15
  • Peer Reviewed
• [Journal Article] Dysregulation of systemic iron metabolism in alcoholic liver diseases. (2008)
  • Author(s): Kohgo Y, Ohtake T, Ikuta K, Suzuki Y, Torimoto Y, Kato J
  • Journal Title: J Gastroenterol Hepatol. 23 Suppl 1 Pages: S78-81
  • Peer Reviewed
• [Presentation] Diferric Transferrin-Sensed Hepcidin Upregulation in Human Hepatoma-Derived Cell Line Is Differentially Controlled in Each Isoform 20, 22, 25, Confirmation by a Novel Simultaneous Quantification by Liquid Chromatography/Tandem Mass Spectrometry. (2009)
  • Author(s): Ikuta K, Hosoki T, Shimonaka, Sasaki Y, Yasuno H, Ohtake T, Sasaki K, Torimoto Y, Saito K, Kohgo Y
  • Organizer: The American Society of Hematology. 51th Annual Meeting and Exposition.
  • Place of Presentation: New Orleans LA, USA
  • Year and Date: 20091205-20091208
• [Presentation] Changes of the expression of the genes involved in iron metabolism by the iron chelation therapy and phrebotomy in the iron overloaded mouse model. (2009)
  • Author(s): Hosoki T, Ikuta K, Okamura N, Shindo M, Sato K, Ohtake T, Sasaki K, Torimoto Y, Kohgo Y
  • Organizer: The Third Congress of the International BioIron Society (BioIron 2009)
  • Place of Presentation: Porto, Portugal
  • Year and Date: 20090607-20090611
• [Presentation] A novel simultaneous quantification of hepcidin isoforms by liquid chromatography-mass spectrometry. (2009)
  • Author(s): Ikuta K, Shimonaka Y, Hosoki T, Sasaki Y, Yasuno H, Okamura N, Shindo M, Ohtake T, Sasaki K, Torimoto Y, Sato K, Kohgo Y
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都(優秀演題)
  • Year and Date: 2009-10-25
• [Presentation] 鉄過剰マウスモデルにおける鉄キレート療法および瀉血療法による鉄代謝関連分子の変化の検討 (2009)
  • Author(s): 細木卓明,生田克哉,伊藤巧,岡村直香,進藤基博,佐藤一也,大竹孝明,佐々木勝則,鳥本悦宏,高後裕
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都(優秀演題)
  • Year and Date: 2009-10-25
• [Presentation] Changes of the expressions of the genes involved in iron metabolism by iron chelation therapy in iron overloaded mouse model. (2008)
  • Author(s): Ikuta K, Torimoto Y, Hosoki T, Jimbo J, Shindo M, Sato K, Ohtake T, Sasaki K, Kohgo Y
  • Organizer: The American Society of Hematology. 50th Annual Meeting and Exposition.
  • Place of Presentation: San Francisco CA, USA
  • Year and Date: 20081206-20081209
• [Presentation] Symposium; Heme and Iron Metabolism. Body iron transport for erythropoiesis. (2008)
  • Author(s): 鳥本悦宏
  • Organizer: The 8th International Porphyrin-Heme Symposium.
  • Place of Presentation: Matsue, Shimane, Japan
  • Year and Date: 20081016-20081017
• [Remarks] ホームページ等