Evaluation of the neurological substrates for stress induced oral dysesthesia.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20592171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Gifu University
• Principal Investigator: ITOH Kazuo Gifu University, 医学系研究科, 教授 (60093184)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Hiroyuki 岐阜大学, 医学系研究科, 准教授 (10211434)
• Project Period (FY): 2008 – 2010
• Keywords: ストレス / 情動 / 口腔感覚 / 三叉神経中脳路核 / 扁桃体 / 青斑核 / 脳 / 神経

Research Abstract

The central nucleus of amygdala and the locus coeruleus are proved to send direct projections to the mesencephalic trigeminal nucleus. Injections of neuronal tract tracers (biotinylated dextran amine, Fast blue) revealed that limited injections of Fast blue into the mesencephalic trigeminal nucleus resulted in retrogradely labeled cells in the central amygdaloid nucleus. Orthograde labeling of the central amygdaloid nucleus resulted in terminal swellings in the mesencephalic trigeminal nucleus. Electron microscopic observation confirmed these findings. Selective cellular labeling of the locus coeruleus using biotinilated dextran amine revealed that the axons of locus coeruleus neurons were followed directly to the mesencephalic trigeminal nucleus gangion cells. The results suggest that emotion and stress directly modify proprioceptive signals from periodontal ligament and masticatory muscles.

Research Products

• [Journal Article] Direct projections from the central amygdaloid nucleus to the mesencephalic trigeminal nucleus in rats. (2011)
  • Author(s): Shirasu, M, Takahashi, T., Yamamoto, T., Itoh, K., Sato, S., Nakamura, H.
  • Journal Title: Brain Research 1400(in press)
  • Peer Reviewed
• [Journal Article] The locus coeruleus projects to the mesencephalic trigeminal nucleus in rats. (2010)
  • Author(s): Takahashi, T., Shirasu, M., Shirasu, M., Kubo, K., Onozuka, M., Sato, S., Itoh, K., Nakamura, H.
  • Journal Title: Neuroscience Research 68 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Dynamic properties of the representation of the visual field midline in the visual areas 17 and 18 of the ferret(Mustela putorius). (2008)
  • Author(s): Nakamura, H., Chaumon, M., Klijn, F., Innocenti, G.M.
  • Journal Title: Cerebral Cortex 18 Pages: 1941-1950
  • Peer Reviewed
• [Presentation] Amygdala projection to the primary sensory nucleus. (2009)
  • Author(s): 中村浩幸, 白数正義, 伊藤和夫
  • Organizer: 第32回日本神経科学大会.SY2-C3-1
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] The amygdala : at the crossroad of self and other. (2009)
  • Author(s): Rockland, K.S., Nakamura H.(Chair persons).
  • Organizer: 第32回日本神経科学大会.SY2-C3
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] ラットにおける扁桃体と三叉神経中脳路核の線維連絡 (2009)
  • Author(s): 白数正義、高橋豪、山本利春、白数真理、伊藤和夫、小野塚実、佐藤貞雄、中村浩幸
  • Organizer: 第32回日本神経科学大会.P2-i09
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] 扁桃体と三叉神経中脳路核:情動感覚連関 (2009)
  • Author(s): 中村浩幸、白数正義、高橋豪、白数真理、伊藤和夫
  • Organizer: 第114回日本解剖学会総会・全国学術集会.S05-2
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-28
• [Presentation] Cortical connections of visual area V3A in the macaque. (2008)
  • Author(s): Nakamura H., Koketsu, D., Shirasu M., Takahashi T., Shirasu M., Mikami, A., Itoh K.
  • Organizer: 6^<th> Forum of European Neuroscience.
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2008-07-15
• [Presentation] Projection from medial raphe to mesencephalic trigeminal nucleus. (2008)
  • Author(s): 白数真理, 高橋豪, 白数正義, 中村浩幸, 伊藤和夫, 佐藤貞雄
  • Organizer: The International Association for Dental Research 86^<th> General Session & Exhibition. #1408
  • Place of Presentation: Tronto, Ontario, Canada
  • Year and Date: 2008-07-03
• [Presentation] Single axon tracing from locus coeruleus to mesencephalic trigeminal nucleus. (2008)
  • Author(s): 高橋豪, 白数真理, 白数正義, 中村浩幸, 伊藤和夫, 佐藤貞雄
  • Organizer: The International Association for Dental Research 86^<th> General Session & Exhibition. #1409
  • Place of Presentation: Tronto, Ontario, Canada
  • Year and Date: 2008-07-03
• [Book] Neuranatomy Research Advances(Visual Cortex : Crosstalk and Concomitant Communications between Extrastriate Visual Areas.)(Flynn, C.E., Callaghan, B.R.eds.). (2010)
  • Author(s): Nakamura, H., Itoh, K.
  • Total Pages: 145-165
  • Publisher: Nova Science Publishers
• [Book] Novel Trands in Brain Science(Involvement of Dysfunctional Mastication in Cognitive System Deficits in the Mouse.)(Onozuka. M., Yen, C.T.eds.). (2008)
  • Author(s): Watanabe, K., Kubo, K., Nakamura, H., Tachibana, A., Kim, W., Ono, Y., Sasaguri, K., Onozuka, M.
  • Total Pages: 115-129
  • Publisher: Springer
• [Remarks] ホームページ等