2010 Fiscal Year Final Research Report

Investigation of the mechanisms of osteogenic differentiation by RhoA in periodontal ligament cells for cell transplantation

Research Project

Project/Area Number	20592429
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Periodontal dentistry
Research Institution	Okayama University
Principal Investigator	YAMAMOTO Tadashi Okayama University, 岡山大学病院, 講師 (50432662)
Co-Investigator(Kenkyū-buntansha)	TAKASHIBA Shogo 岡山大学, 大学院・医歯薬学総合研究科, 教授 (50226768) MINESHIBA Junji 岡山大学, 岡山大学病院, 講師 (00509383) YAMASHIRO Keisuke 岡山大学, 大学院・医歯薬学総合研究科, 助教 (30581087) NARUISHI Koji 岡山大学, 岡山大学病院, 講師 (00346446) SHIOMI Nobuyuki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (90432632)
Co-Investigator(Renkei-kenkyūsha)	SONOYAMA Wataru 岡山大学, 岡山大学病院, 助教 (40325121)
Project Period (FY)	2008 – 2010
Keywords	歯根膜 / 細胞分化 / RhoA-ROCKシグナル / Y-27632 / 遺伝子導入
Research Abstract	In periodontal ligament cells (PDL cells), cytoskeletal signaling regulated by Rho-ROCK is critical in the differentiation process, and the expression of osteogenic genes such as BMP-4, Wnt3a, and Wnt5a. Meanwhile over-expression of RhoA or ROCK showed significant low cell proliferation, suggesting that PDL cells differentiation is coordinately regulated by Rho-ROCK induced cytoskeletal molecules, as well as soluble growth factors and extracellular matrix.

(3 results)