2023 Fiscal Year Final Research Report
Genetic manipulation of chicken for better vaccine production
| Project/Area Number |
20H02537
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
金岡 英徳 愛知工業大学, 工学部, 准教授 (30631973)
小野 悦郎 九州大学, 医学研究院, 教授 (00160903)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | ニワトリ / インフルエンザ / ワクチン / 遺伝子改変 |
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| Outline of Final Research Achievements |
The human influenza virus propagated in embryonated chicken eggs is an "avianized” virus with the risk of mutation. We aimed to improve the propagation of the human virus in eggs by using transgenic chickens. For this purpose, it was necessary to create two transgenic chickens to increase safety. We implanted the transfected primordial germ cells into recipient embryos and succeeded in obtaining two transgenic chickens that we planned to create. In order to improve the efficiency of transgenic chicken production, we examined the characteristics of chicken primordial germ cells and found that strong expression of a glycosyltransferase suppresses the proliferation of the cells.
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| Free Research Field |
ニワトリの生殖工学
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| Academic Significance and Societal Importance of the Research Achievements |
作製した2系統のトランスジェニックニワトリの交配を開始しており、今後得られた卵を解析することで糖鎖変換とインフルエンザウイルスの増殖を評価する必要がある。これにより、期待通りインフルエンザワクチン生産に適した鶏卵作製を証明できれば、増殖が難しいワクチン株の生産等へ適用可能な技術となる。また、ニワトリ始原生殖細胞の増殖に関わる複数の因子を見出したことから、今後増殖制御や育種への利用への展開が期待される。
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